TLN2 Orthologs

Aliases: talin 2, KIAA0320, TLN2, DKFZp451B1011, DKFZp686I0976, DKFZp686K0979, ILWEQ

Pfam Domain Structure

From TLN2 (human)

Members of the TLN2 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB TLN2 Tln2 Tln2 LOC415374 rhea Y71G12B.11
Entrez Gene 83660 70549 315776 415374 38978 171736
Uniprot TLN2_HUMAN TLN2_MOUSE - - - -
OMIM 607349 - - - - -
Reagents - - - - - -
Results 4 available - - - 5 available 1 available
References
  1. Abrams CS. Intracellular signaling in platelets. Curr. Opin. Hematol. 2005; 12:401-5 (PubMed)
  2. Burridge K, Mangeat P. An interaction between vinculin and talin. Nature 1984; 308:744-6 (PubMed)
  3. Calderwood DA. Talin controls integrin activation. Biochem. Soc. Trans. 2004; 32:434-7 (PubMed)
  4. Campbell ID, Ginsberg MH. The talin-tail interaction places integrin activation on FERM ground. Trends Biochem. Sci. 2004; 29:429-35 (PubMed)
  5. Cram EJ, Schwarzbauer JE. The talin wags the dog: new insights into integrin activation. Trends Cell Biol. 2004; 14:55-7 (PubMed)
  6. Critchley DR. Biochemical and structural properties of the integrin-associated cytoskeletal protein talin. Annu Rev Biophys 2009; 38:235-54 (PubMed)
  7. Critchley DR. Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion. Biochem. Soc. Trans. 2004; 32:831-6 (PubMed)
  8. Critchley DR. Genetic, biochemical and structural approaches to talin function. Biochem. Soc. Trans. 2005; 33:1308-12 (PubMed)
  9. del Rio A, Perez-Jimenez R, Liu R, Roca-Cusachs P, Fernandez JM, Sheetz MP. Stretching single talin rod molecules activates vinculin binding. Science 2009; 323:638-41 (PubMed)
  10. Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, Huttenlocher A. Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat. Cell Biol. 2004; 6:977-83 (PubMed)
  11. Hemmings L, Rees DJ, Ohanian V, Bolton SJ, Gilmore AP, Patel B, Priddle H, Trevithick JE, Hynes RO, Critchley DR. Talin contains three actin-binding sites each of which is adjacent to a vinculin-binding site. J. Cell. Sci. 1996; 109 ( Pt 11):2715-26 (PubMed)
  12. Horwitz A, Duggan K, Buck C, Beckerle MC, Burridge K. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage. Nature 1986; 320:531-3 (PubMed)
  13. Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol. Rev. 2008; 88:489-513 (PubMed)
  14. Lin J, Miller MJ, Shaw AS. The c-SMAC: sorting it all out (or in). J. Cell Biol. 2005; 170:177-82 (Full Text)
  15. Moes M, Rodius S, Coleman SJ, Monkley SJ, Goormaghtigh E, Tremuth L, Kox C, van der Holst PP, Critchley DR, Kieffer N. The integrin binding site 2 (IBS2) in the talin rod domain is essential for linking integrin beta subunits to the cytoskeleton. J. Biol. Chem. 2007; 282:17280-8 (PubMed)
  16. Nayal A, Webb DJ, Horwitz AF. Talin: an emerging focal point of adhesion dynamics. Curr. Opin. Cell Biol. 2004; 16:94-8 (PubMed)
  17. Ratnikov BI, Partridge AW, Ginsberg MH. Integrin activation by talin. J. Thromb. Haemost. 2005; 3:1783-90 (PubMed)
  18. Watanabe N, Bodin L, Pandey M, Krause M, Coughlin S, Boussiotis VA, Ginsberg MH, Shattil SJ. Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin alphaIIbbeta3. J. Cell Biol. 2008; 181:1211-22 (Full Text)
Consortium Results & Data
Discovery» C. elegans genes affecting migration
Gene Description/Function Parental P0 defect Distribution1
(percent)
Progeny F1 defect Distribution2
(percent)
Express DTC Express BWM
Gene: Y71G12B.11 (Y71G12B.11)
Gene Pair ID
CMKB
Entrez Gene
Y71G12B.11a
AhringerAutoClass: Unknown
Function: Cell Architecture
0.98 (N=52)
0.9 – 1
Type 1: 94
Type 2: 0
Type 3: 6
   
Yes
Yes
Discovery» Genes encoding cytoskeleton-associated proteins enriched in migratory cells
Gene Description Nearest mammalian (Human) homolog Fold Change
rhea
GeneID:38978
Entrez
Flybase
Rhea
Proposed Function: Links membrane receptors to the F-actin cytoskeleton
Conserved Protein Domains: Band 4.1, FERM-C, PTB, ILWEQ
TLN2
GeneID: 83660
CMKB Orthologs
1.78
Discovery» Migratory cell enriched genes encoding proteins associated with vesicle traffic
Gene Description Nearest mammalian (Human) homolog Cellular component(s) Fold Change
rhea
GeneID:38978
Entrez
Flybase
Rhea
Proposed Function: Links membrane receptors to the F-actin cytoskeleton
TLN2
GeneID: 83660
CMKB Orthologs
none
1.78
Discovery» Drosophila Migration Genes
Gene Name Function Cell
Type
Phenotypes Reagents/
Alleles
Reference
rhea
Entrez: CG6831
Flybase: FBgn0020254
rhea cytoskeletal anchoring, talin homolog embryonic trachea Loss of function: fail to migrate
Autonomous: autonomous
rhea1, rhea2 Levi, 2006
Discovery» wt-migratory_cells vs wt-non-migratory_cells
Symbol Description Median Values Fold Change Probe ID
rhea
Flybase
Entrez
CG6831 gene product from transcript CG6831-RA Mig: 762.8
Non-Mig: 772.9
0.98697 151754_at
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG6831
Entrez Gene: 38978
rhea
Aliases: tendrils; talin; Talin
7.500
1.599
0.825 1.07 Actin: 1.313
Area: 0.645
Ratio: 2.036
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
TLN2
Aliases: ILWEQ
Entrez Gene: 83660
siRNA catalog
talin 2
mRNA: NM_015059
Library: MAR
Classification: cytoskeletal
Final bin: Discordant
SMARTpool bin: Low Alamar
Avg area: 2.56
Avg Alamar: 0.55
Knockdown %: not done
ERBB2: Low Alamar
BCL2: Low Alamar
TLN2
Link
TLN2
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
TLN2
Aliases: ILWEQ
Entrez Gene: 83660
siRNA catalog
talin 2
mRNA: NM_015059
Library: MAR
TLN2
Link
TLN2
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00219299 TLN2 TALIN 2. 7 5 21 16 3.00
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
IPI Locus Description CB Spectra PD Spectra ratio Location
IPI00219299 TLN2 TALIN 2. 7 21 3 cytoskeleton