TLN2 Orthologs
Aliases: talin 2, KIAA0320, TLN2, DKFZp451B1011, DKFZp686I0976, DKFZp686K0979, ILWEQ
Pfam Domain Structure
From TLN2 (human)
Members of the TLN2 ortholog set
| Human | Mouse | Rat | Chicken | Fly | Worm | |
| CMKB | TLN2 | Tln2 | Tln2 | LOC415374 | rhea | Y71G12B.11 |
| Entrez Gene | 83660 | 70549 | 315776 | 415374 | 38978 | 171736 |
| Uniprot | TLN2_HUMAN | TLN2_MOUSE | - | - | - | - |
| OMIM | 607349 | - | - | - | - | - |
| Reagents | - | - | - | - | - | - |
| Results | 4 available | - | - | - | 5 available | 1 available |
References
- Abrams CS. Intracellular signaling in platelets. Curr. Opin. Hematol. 2005; 12:401-5 (PubMed)
- Burridge K, Mangeat P. An interaction between vinculin and talin. Nature 1984; 308:744-6 (PubMed)
- Calderwood DA. Talin controls integrin activation. Biochem. Soc. Trans. 2004; 32:434-7 (PubMed)
- Campbell ID, Ginsberg MH. The talin-tail interaction places integrin activation on FERM ground. Trends Biochem. Sci. 2004; 29:429-35 (PubMed)
- Cram EJ, Schwarzbauer JE. The talin wags the dog: new insights into integrin activation. Trends Cell Biol. 2004; 14:55-7 (PubMed)
- Critchley DR. Biochemical and structural properties of the integrin-associated cytoskeletal protein talin. Annu Rev Biophys 2009; 38:235-54 (PubMed)
- Critchley DR. Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion. Biochem. Soc. Trans. 2004; 32:831-6 (PubMed)
- Critchley DR. Genetic, biochemical and structural approaches to talin function. Biochem. Soc. Trans. 2005; 33:1308-12 (PubMed)
- del Rio A, Perez-Jimenez R, Liu R, Roca-Cusachs P, Fernandez JM, Sheetz MP. Stretching single talin rod molecules activates vinculin binding. Science 2009; 323:638-41 (PubMed)
- Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, Huttenlocher A. Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat. Cell Biol. 2004; 6:977-83 (PubMed)
- Hemmings L, Rees DJ, Ohanian V, Bolton SJ, Gilmore AP, Patel B, Priddle H, Trevithick JE, Hynes RO, Critchley DR. Talin contains three actin-binding sites each of which is adjacent to a vinculin-binding site. J. Cell. Sci. 1996; 109 ( Pt 11):2715-26 (PubMed)
- Horwitz A, Duggan K, Buck C, Beckerle MC, Burridge K. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage. Nature 1986; 320:531-3 (PubMed)
- Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol. Rev. 2008; 88:489-513 (PubMed)
- Lin J, Miller MJ, Shaw AS. The c-SMAC: sorting it all out (or in). J. Cell Biol. 2005; 170:177-82 (PubMed)
- Moes M, Rodius S, Coleman SJ, Monkley SJ, Goormaghtigh E, Tremuth L, Kox C, van der Holst PP, Critchley DR, Kieffer N. The integrin binding site 2 (IBS2) in the talin rod domain is essential for linking integrin beta subunits to the cytoskeleton. J. Biol. Chem. 2007; 282:17280-8 (PubMed)
- Nayal A, Webb DJ, Horwitz AF. Talin: an emerging focal point of adhesion dynamics. Curr. Opin. Cell Biol. 2004; 16:94-8 (PubMed)
- Ratnikov BI, Partridge AW, Ginsberg MH. Integrin activation by talin. J. Thromb. Haemost. 2005; 3:1783-90 (PubMed)
- Watanabe N, Bodin L, Pandey M, Krause M, Coughlin S, Boussiotis VA, Ginsberg MH, Shattil SJ. Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin alphaIIbbeta3. J. Cell Biol. 2008; 181:1211-22 (PubMed)
Consortium Results & Data
Discovery» Genes encoding cytoskeleton-associated proteins enriched in migratory cells
| Gene | Description | Nearest mammalian (Human) homolog | Fold Change |
|
rhea GeneID:38978 Entrez Flybase |
Rhea Proposed Function: Links membrane receptors to the F-actin cytoskeleton Conserved Protein Domains: Band 4.1, FERM-C, PTB, ILWEQ |
TLN2 GeneID: 83660 CMKB Orthologs |
1.78
|
Discovery» Migratory cell enriched genes encoding proteins associated with vesicle traffic
| Gene | Description | Nearest mammalian (Human) homolog | Cellular component(s) | Fold Change |
|
rhea GeneID:38978 Entrez Flybase |
Rhea Proposed Function: Links membrane receptors to the F-actin cytoskeleton |
TLN2 GeneID: 83660 CMKB Orthologs |
none
|
1.78
|
Discovery» Drosophila Migration Genes
| Gene | Name | Function |
Cell Type |
Phenotypes |
Reagents/ Alleles |
Reference |
|
rhea Entrez: CG6831 Flybase: FBgn0020254 |
rhea | cytoskeletal anchoring, talin homolog | embryonic trachea |
Loss of function: fail to migrate Autonomous: autonomous |
rhea1, rhea2 | Levi, 2006 |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
rhea Flybase Entrez |
CG6831 gene product from transcript CG6831-RA |
Mig: 762.8 Non-Mig: 772.9 |
0.98697 | 151754_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG6831 Entrez Gene: 38978 |
rhea Aliases: tendrils; talin; Talin |
7.500 1.599 |
0.825 | 1.07 |
Actin: 1.313 Area: 0.645 Ratio: 2.036 |
|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
TLN2 Aliases: ILWEQ Entrez Gene: 83660 siRNA catalog |
talin 2 mRNA: NM_015059 Library: MAR Classification: cytoskeletal |
Final bin: Discordant SMARTpool bin: Low Alamar Avg area: 2.56 Avg Alamar: 0.55 Knockdown %: not done |
ERBB2: Low Alamar BCL2: Low Alamar |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
TLN2 Aliases: ILWEQ Entrez Gene: 83660 siRNA catalog |
talin 2 mRNA: NM_015059 Library: MAR |
 Link |
 Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00219299 | TLN2 | TALIN 2. | 7 | 5 | 21 | 16 | 3.00 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00219299 | TLN2 | TALIN 2. | 7 | 21 | 3 | cytoskeleton |
Discovery» C. elegans genes affecting migration
| Gene | Description/Function | Parental P0 defect |
Distribution1 (percent) |
Progeny F1 defect |
Distribution2 (percent) |
Express DTC | Express BWM |
|
Gene: Y71G12B.11 (Y71G12B.11) Gene Pair ID CMKB Entrez Gene |
Y71G12B.11a AhringerAutoClass: Unknown Function: Cell Architecture |
0.98 (N=52) 0.9 – 1 |
Type 1: 94 Type 2: 0 Type 3: 6 |
Yes
|
Yes
|