TLN1 Orthologs

Aliases: talin 1, TLN, TLN1, ILWEQ, KIAA1027

Pfam Domain Structure

From TLN1 (human) - phosphorylation sites added via direct mapping

Members of the TLN1 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB TLN1 Tln1 Tln1 TLN1 - -
Entrez Gene 7094 21894 313494 395194 - -
Uniprot TLN1_HUMAN TLN1_MOUSE - TLN1_CHICK - -
OMIM 186745 - - - - -
Reagents 9 available 11 available - 5 available - -
Results 4 available 1 available - - - -
References
  1. Abrams CS. Intracellular signaling in platelets. Curr. Opin. Hematol. 2005; 12:401-5 (PubMed)
  2. Burridge K, Mangeat P. An interaction between vinculin and talin. Nature 1984; 308:744-6 (PubMed)
  3. Calderwood DA. Talin controls integrin activation. Biochem. Soc. Trans. 2004; 32:434-7 (PubMed)
  4. Campbell ID, Ginsberg MH. The talin-tail interaction places integrin activation on FERM ground. Trends Biochem. Sci. 2004; 29:429-35 (PubMed)
  5. Cram EJ, Schwarzbauer JE. The talin wags the dog: new insights into integrin activation. Trends Cell Biol. 2004; 14:55-7 (PubMed)
  6. Critchley DR. Biochemical and structural properties of the integrin-associated cytoskeletal protein talin. Annu Rev Biophys 2009; 38:235-54 (PubMed)
  7. Critchley DR. Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion. Biochem. Soc. Trans. 2004; 32:831-6 (PubMed)
  8. Critchley DR. Genetic, biochemical and structural approaches to talin function. Biochem. Soc. Trans. 2005; 33:1308-12 (PubMed)
  9. del Rio A, Perez-Jimenez R, Liu R, Roca-Cusachs P, Fernandez JM, Sheetz MP. Stretching single talin rod molecules activates vinculin binding. Science 2009; 323:638-41 (PubMed)
  10. Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, Huttenlocher A. Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat. Cell Biol. 2004; 6:977-83 (PubMed)
  11. Hemmings L, Rees DJ, Ohanian V, Bolton SJ, Gilmore AP, Patel B, Priddle H, Trevithick JE, Hynes RO, Critchley DR. Talin contains three actin-binding sites each of which is adjacent to a vinculin-binding site. J. Cell. Sci. 1996; 109 ( Pt 11):2715-26 (PubMed)
  12. Horwitz A, Duggan K, Buck C, Beckerle MC, Burridge K. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage. Nature 1986; 320:531-3 (PubMed)
  13. Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol. Rev. 2008; 88:489-513 (PubMed)
  14. Lin J, Miller MJ, Shaw AS. The c-SMAC: sorting it all out (or in). J. Cell Biol. 2005; 170:177-82 (Full Text)
  15. Moes M, Rodius S, Coleman SJ, Monkley SJ, Goormaghtigh E, Tremuth L, Kox C, van der Holst PP, Critchley DR, Kieffer N. The integrin binding site 2 (IBS2) in the talin rod domain is essential for linking integrin beta subunits to the cytoskeleton. J. Biol. Chem. 2007; 282:17280-8 (PubMed)
  16. Nayal A, Webb DJ, Horwitz AF. Talin: an emerging focal point of adhesion dynamics. Curr. Opin. Cell Biol. 2004; 16:94-8 (PubMed)
  17. Ratnikov BI, Partridge AW, Ginsberg MH. Integrin activation by talin. J. Thromb. Haemost. 2005; 3:1783-90 (PubMed)
  18. Watanabe N, Bodin L, Pandey M, Krause M, Coughlin S, Boussiotis VA, Ginsberg MH, Shattil SJ. Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin alphaIIbbeta3. J. Cell Biol. 2008; 181:1211-22 (Full Text)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
TLN1 none phosphorylation site Talin Endogenous from human platelet 30 phospho-sites in 95.1% coverage of 2540 aa Ginsberg Ratnikov et al.
TLN1 none structure

Talin rod

X-Ray crystallography

completed Liddington Patel et al.
TLN1 none structure

Talin-rod domain and actin

EM and helical reconstruction

completed Hanein
-
TLN1 none structure

Talin (full length)

X-Ray crystallography

completed Liddington
-
TLN1 none structure

Talin rod 655-911

NMR

completed Critchley Patel et al.
TLN1 none structure

Talin fragments for protein splicing and chemical labeling

Cell-based studies

completed Liddington
-
TLN1 none structure

Talin C-terminal helix required for dimer formation

X-Ray crystallography

completed Critchley Gingras et al., Gingras et al.
TLN1 none structure

Talin head for head-tail binding studies

Calorimetric

completed Liddington
-
TLN1 none structure Talin (Ferm-domain) and Actin EM and helical reconstruction completed Hanein Lee et al.
TLN1 none structure

Vinculin and Talin

Atomic Force Microscope

completed Liddington
-
TLN1 none structure

Talin

2D crystallization on lipid monolayers

completed Taylor
-
TLN1 none structure

Alpha-actinin / beta1-integrin - vinculin 1-258 - talin FERM domain complex

2D crystallization on lipid monolayers

completed Taylor
-
TLN1 none structure

Beta tails as an interaction hub

NMR

completed Campbell Oxley et al.
TLN1 none structure

Talin C-terminal actin binding site (I/LWEQ motif)

NMR

completed Critchley Gingras et al., Goult et al.
TLN1 none structure

Talin FO/F1(1-200)

NMR

completed Critchley Gingras et al.
TLN1 none structure Layilin-talin (196-400) X-Ray crystallography, NMR completed Liddington Wegener et al.
TLN1 none structure PIPKIg -talin (196-400) X-Ray crystallography, NMR completed Liddington de Pereda et al.
TLN1 none structure

Talin -beta3 integrin

X-Ray crystallography, NMR

data available Liddington GarcĂ­a-Alvarez et al., Wegener et al., Calderwood et al.
TLN1 none structure

Talin (C-terminal Actin-binding domain)

EM, SAXS, docking and helical reconstruction

completed Hanein Gingras et al., Bate et al., Goult et al., Goult et al.
TLN1 none structure

Full-length talin and actin

EM and tomography

completed Hanein Goult et al.
TLN1 none structure

Talin-vinculin

2D crystallization on lipid monolayers

completed Taylor
-

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Consortium Related Products*
Mice » activity page
Ortholog Species Name Method Phenotype Status Jax Availability Source Publications
TLN1 mouse Talin 1
-
Embryonic lethality at E8.5-9.5 with disorganization of embryos at gastrulation. completed
-
-
Monkley et al.
TLN1 mouse Talin - trophoblast cells
-
Reduced spreading on fibronectin and laminin. completed
-
-
Monkley et al.
Constructs » activity page
Ortholog Species Targets Name Amino Acid Range Portion Protocol Source
TLN1 human Talin / PIPKIg - soluble pET30a-talin 1-2541 Talin-full length, S429D gain-of-function mutant
-
Liddington
TLN1 human Talin / PIPKIg pET30b-talin rod 397-2541 Talin rod available Liddington
TLN1 human Talin / PIPKIg - soluble pET30a-talin 1-2541 Full length Talin available Liddington
TLN1 human Talin / PIPKIg pET151-DTOPO talin 196-889 196-889 Talin FERM F2F3 + part of rod available
-
TLN1 human Talin / PIPKIg - soluble pET30a-talin 1-2541 Talin-full length, S446D gain-of-function mutant
-
Liddington
TLN1 human Talin / PIPKIg - soluble pET30a-talin 1-2541 Talin-full length, S429D/S446D double gain-of-function mutant
-
Liddington
TLN1 human Talin / PIPKIg pGEX-4T-2 1984-2344 Talin VBS3
-
-
TLN1 human Talin / PIPKIg pET-29+ Talin Fragment G (L2094A/I12095A mutant)
-
-
TLN1 human Talin / PIPKIg pGEX-4T-2 2071-2135 Talin's actin-binding domain
-
-
TLN1 mouse Layilin / Talin pET15b-layilin 207-400 Layilin tail+talin F2+F3 fusion protein
-
Liddington
TLN1 mouse Talin / PIPKIg - soluble/great yield pET15b-talin 482-636 482-636 Talin's Vinculin-binding domain (VBS1; 4-helix bundle) available Liddington
TLN1 mouse Talin / PIPKIg - soluble/great yield pET15b-talin 482-655 482-655 Talin's VBS1 (autoinhibited 5-helix bundle) available Liddington
TLN1 mouse Talin / PIPKIg - soluble/great yield pET15b-talin 482-789 482-789 Talin rod fragment available Liddington
TLN1 mouse Talin / PIPKIg - soluble/great yield pET151-DTOPO talin 482-889 482-889 Talin rod fragment available Liddington
TLN1 mouse Talin / PIPKIg - soluble/great yield pET151-DTOPO talin 482-889TTTT 482-889 Talin 482-889 stabilized fragment quadruple mutant (T775V/T809I/T833I/T867V) available Liddington
TLN1 mouse Talin / PIPKIg - soluble/great yield pET15b-talin 755-889 755-889 Talin's Vinculin-binding domain 2 (VBS2) available Liddington
TLN1 mouse Talin / PIPKIg - soluble/great yield pET15b-talin 1647-2541 1647-2541 Talin C-terminal fragment available Liddington
TLN1 mouse Talin / PIPKIg - soluble/good yield pET151-DTOPO talin 1843-1973 1843-1973 Talin VBS2 available Liddington
TLN1 chicken Talin / PIPKIg - soluble/great yield pET15b-His-F2F3short 209-400 Talin F2F3 fused to PIPKIgamma tail
-
Liddington
TLN1 chicken Talin - soluble/ok yield pET15b-His-Talin Head 1-400 Talin Head domain available Liddington
TLN1 chicken Talin - soluble/great yield pET15b-His-F2F3 196-400 Talin FERM domain (F2F3 portion) available Liddington
TLN1 chicken Talin / PIPKIg - soluble/ok yield pMAL-cgx-F3,4 309-405 Talin FERM F3 portion
-
Liddington
TLN1 chicken Talin / PIPKIg - low yield pET15b-His-F3 309-405 Talin FERM F3 portion available Liddington

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.

Pathways

How many degrees of interaction?

Update
Legend: Legend for CMKB pathways