PAK4 Orthologs

Aliases: p21-activated kinase 4, p21(CDKN1A)-activated kinase 4, protein kinase related to S. cerevisiae STE20, effector for Cdc42Hs, PAK-4, PAK4

Pfam Domain Structure

From PAK4 (human)

Members of the PAK4 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB PAK4 Pak4 Pak4 - - -
Entrez Gene 10298 70584 292756 - - -
Uniprot PAK4_HUMAN PAK4_MOUSE - - - -
OMIM 605451 - - - - -
Reagents - - - - - -
Results 5 available - - - - -
References
  1. Bokoch GM. Biology of the p21-activated kinases. Annu. Rev. Biochem. 2003; 72:743-81 (PubMed)
  2. Chew TL, Masaracchia RA, Goeckeler ZM, Wysolmerski RB. Phosphorylation of non-muscle myosin II regulatory light chain by p21-activated kinase (gamma-PAK). J. Muscle Res. Cell. Motil. 1998; 19:839-54 (PubMed)
  3. Cotteret S, Chernoff J. Pak GITs to Aurora-A. Dev. Cell 2005; 9:573-4 (PubMed)
  4. Dummler B, Ohshiro K, Kumar R, Field J. Pak protein kinases and their role in cancer. Cancer Metastasis Rev. 2009; 28:51-63 (PubMed)
  5. Fryer BH, Field J. Rho, Rac, Pak and angiogenesis: old roles and newly identified responsibilities in endothelial cells. Cancer Lett. 2005; 229:13-23 (PubMed)
  6. Gerthoffer WT. Actin cytoskeletal dynamics in smooth muscle contraction. Can. J. Physiol. Pharmacol. 2005; 83:851-6 (PubMed)
  7. Hoefen RJ, Berk BC. The multifunctional GIT family of proteins. J. Cell. Sci. 2006; 119:1469-75 (PubMed)
  8. Hofmann C, Shepelev M, Chernoff J. The genetics of Pak. J. Cell. Sci. 2004; 117:4343-54 (PubMed)
  9. Howe AK. Regulation of actin-based cell migration by cAMP/PKA. Biochim. Biophys. Acta 2004; 1692:159-74 (PubMed)
  10. Juliano RL, Reddig P, Alahari S, Edin M, Howe A, Aplin A. Integrin regulation of cell signalling and motility. Biochem. Soc. Trans. 2004; 32:443-6 (PubMed)
  11. Labruyère E, Guillén N. Host tissue invasion by Entamoeba histolytica is powered by motility and phagocytosis. Arch. Med. Res. 2006; 37:253-8 (PubMed)
  12. Manser E, Leung T, Salihuddin H, Zhao ZS, Lim L. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 1994; 367:40-6 (PubMed)
  13. Parrini MC, Matsuda M, de Gunzburg J. Spatiotemporal regulation of the Pak1 kinase. Biochem. Soc. Trans. 2005; 33:646-8 (PubMed)
  14. Settleman J. PAK-in' up cGMP for the move. Cell 2007; 128:237-8 (PubMed)
  15. Vlahou G, Rivero F. Rho GTPase signaling in Dictyostelium discoideum: insights from the genome. Eur. J. Cell Biol. 2006; 85:947-59 (PubMed)
  16. Zhao ZS, Manser E. PAK and other Rho-associated kinases--effectors with surprisingly diverse mechanisms of regulation. Biochem. J. 2005; 386:201-14 (Full Text)
Consortium Results & Data
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
PAK4
Entrez Gene: 10298
siRNA catalog
p21(CDKN1A)-activated kinase 4
mRNA: NM_005884
Library: Kinase
Classification: Atypical Kinase
Final bin: No Phenotype
SMARTpool bin: No change
Avg area: 1.63
Avg Alamar: 1.05
Knockdown %: 80-90
  PAK4
Link
PAK4
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
PAK4
Entrez Gene: 10298
siRNA catalog
p21(CDKN1A)-activated kinase 4
mRNA: NM_005884
Library: Kinase
PAK4
Link
PAK4
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00014068 PAK4 SPLICE ISOFORM 1 OF SERINE/THREONINE-PROTEIN KINASE PAK 4. 3 3 7 3 2.33
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
IPI Locus Description CB Spectra PD Spectra ratio Location
IPI00014068 PAK4 SPLICE ISOFORM 1 OF SERINE/THREONINE-PROTEIN KINASE PAK 4. 3 7 2.33 Golgi
Proteomics» Table 3. Quantitation of phosphopeptides identified in Pseudopodium (PD) and Cell Body (CB) fractions
IPI Locus Description peptide pdcb_p pdcb_t ratio Mouse homology Rat homology Zebrafish homology sd Variable sites
IPI00014068 PAK4 SERINE/THREONINE-PROTEIN KINASE PAK 4. S*LVGTPYWMAPELISR 0.7 2.33 0.3 +++ +++ +++ 0.08