PAK1 Orthologs

Aliases: p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), p21-activated kinase 1, STE20 homolog, yeast, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), PAKalpha, PAK1, MGC130000, MGC130001

Processes & Diseases

Processes involved: protrusion

Pfam Domain Structure

From PAK1 (human) - phosphorylation sites added via direct mapping

Members of the PAK1 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB PAK1 Pak1 Pak1 PAK1 Pak pak-1
Entrez Gene 5058 18479 29431 419083 44039 180873
Uniprot PAK1_HUMAN PAK1_MOUSE PAK1_RAT - - PAK1_CAEEL
OMIM 602590 - - - - -
Reagents - - - - - -
Results 2 available 1 available - - 2 available -
References
  1. Bokoch GM. Biology of the p21-activated kinases. Annu. Rev. Biochem. 2003; 72:743-81 (PubMed)
  2. Chew TL, Masaracchia RA, Goeckeler ZM, Wysolmerski RB. Phosphorylation of non-muscle myosin II regulatory light chain by p21-activated kinase (gamma-PAK). J. Muscle Res. Cell. Motil. 1998; 19:839-54 (PubMed)
  3. Cotteret S, Chernoff J. Pak GITs to Aurora-A. Dev. Cell 2005; 9:573-4 (PubMed)
  4. Dummler B, Ohshiro K, Kumar R, Field J. Pak protein kinases and their role in cancer. Cancer Metastasis Rev. 2009; 28:51-63 (PubMed)
  5. Fryer BH, Field J. Rho, Rac, Pak and angiogenesis: old roles and newly identified responsibilities in endothelial cells. Cancer Lett. 2005; 229:13-23 (PubMed)
  6. Gerthoffer WT. Actin cytoskeletal dynamics in smooth muscle contraction. Can. J. Physiol. Pharmacol. 2005; 83:851-6 (PubMed)
  7. Hoefen RJ, Berk BC. The multifunctional GIT family of proteins. J. Cell. Sci. 2006; 119:1469-75 (PubMed)
  8. Hofmann C, Shepelev M, Chernoff J. The genetics of Pak. J. Cell. Sci. 2004; 117:4343-54 (PubMed)
  9. Howe AK. Regulation of actin-based cell migration by cAMP/PKA. Biochim. Biophys. Acta 2004; 1692:159-74 (PubMed)
  10. Juliano RL, Reddig P, Alahari S, Edin M, Howe A, Aplin A. Integrin regulation of cell signalling and motility. Biochem. Soc. Trans. 2004; 32:443-6 (PubMed)
  11. Labruyère E, Guillén N. Host tissue invasion by Entamoeba histolytica is powered by motility and phagocytosis. Arch. Med. Res. 2006; 37:253-8 (PubMed)
  12. Manser E, Leung T, Salihuddin H, Zhao ZS, Lim L. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 1994; 367:40-6 (PubMed)
  13. Parrini MC, Matsuda M, de Gunzburg J. Spatiotemporal regulation of the Pak1 kinase. Biochem. Soc. Trans. 2005; 33:646-8 (PubMed)
  14. Settleman J. PAK-in' up cGMP for the move. Cell 2007; 128:237-8 (PubMed)
  15. Vlahou G, Rivero F. Rho GTPase signaling in Dictyostelium discoideum: insights from the genome. Eur. J. Cell Biol. 2006; 85:947-59 (PubMed)
  16. Zhao ZS, Manser E. PAK and other Rho-associated kinases--effectors with surprisingly diverse mechanisms of regulation. Biochem. J. 2005; 386:201-14 (Full Text)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
PAK1 none binding partner Pak1 Ectopic expression in HEK cells 13 proteins exclusively co-purified with Pak1 Horwitz Mayhew et al.
PAK1 none phosphorylation site Pak1 Ectopic expression in HEK cells 30 phospho-sites in 80.5% coverage of 529 aa Horwitz Mayhew et al.
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
PAK1
Aliases: PAKalpha
Entrez Gene: 5058
siRNA catalog
p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
mRNA: NM_002576
Library: Kinase
Classification: Tyr Kinase
Focal Adhesion Related: Yes
Final bin: Discordant
SMARTpool bin: Low Alamar
Avg area: 2.14
Avg Alamar: 0.79
Knockdown %: 90-100
ERBB2: Impaired
BCL2: Low Alamar
PAK1
Link
PAK1
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
PAK1
Aliases: PAKalpha
Entrez Gene: 5058
siRNA catalog
p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
mRNA: NM_002576
Library: Kinase
PAK1
Link
PAK1
Link
Discovery» Drosophila Migration Genes
Gene Name Function Cell
Type
Phenotypes Reagents/
Alleles
Reference
Pak
Entrez: CG10295
Flybase: FBgn0014001
PAK-kinase protein serine/threonine kinase activity dorsal closure Loss of function: dorsal closure defects
Autonomous: Both amnioserosa and epidermis
Pak20, PakAID (autoinhibitory domain of dPak) Conder, 2004
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG10295
Entrez Gene: 44039
PAK-kinase
Aliases: Pak1 kinase; p21-associated kinase; p-21 activated kinase; p21-activated kinase; Pak kinase; PAK kinase; p21-activated serine/threonine kinase
10.125
1.138
0.99 0.989 Actin: 0.871
Area: 0.948
Ratio: 0.919
Discovery» PyMT invasive data
Gene mRNA Description Expression Ratio
Pak1
Entrez Gene: 18479
mRNA: AA061378
UniGene: Mm.260227
P21 (CDKN1A)-activated kinase 1 2.01
Consortium Related Products*
Biosensors » activity page
Ortholog Species Name Type Source Publications
PAK1 none PAK Generically encoded caged PAK inhibitor Hahn
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* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.

Pathways

How many degrees of interaction?

Update
Legend: Legend for CMKB pathways