PXN Orthologs
Aliases: paxillin, PXN, hCG_1778014, FLJ16691
Processes & Diseases
Processes involved: adhesion
Pfam Domain Structure
From PXN (chicken) - phosphorylation sites added via direct mapping
Members of the PXN ortholog set
| Human | Mouse | Rat | Chicken | Fly | Worm | |
| CMKB | PXN | Pxn | Pxn | PXN | Pax | pxl-1 |
| Entrez Gene | 5829 | 19303 | 360820 | 395832 | 35215 | 175831 |
| Uniprot | PAXI_HUMAN | PAXI_MOUSE | PAXI_RAT | PAXI_CHICK | - | PXL1_CAEEL |
| OMIM | 602505 | - | - | - | - | - |
| Reagents | 2 available | 4 available | - | - | - | - |
| Results | 3 available | - | - | - | 2 available | - |
References
- Chen PW, Kroog GS. Leupaxin is similar to paxillin in focal adhesion targeting and tyrosine phosphorylation but has distinct roles in cell adhesion and spreading. Cell Adh Migr 2010; 4: (PubMed)
- Deakin NO, Turner CE. Paxillin comes of age. J. Cell. Sci. 2008; 121:2435-44 (PubMed)
- Hoefen RJ, Berk BC. The multifunctional GIT family of proteins. J. Cell. Sci. 2006; 119:1469-75 (PubMed)
- Huang C, Jacobson K, Schaller MD. A role for JNK-paxillin signaling in cell migration. Cell Cycle 2004; 3:4-6 (PubMed)
- Rathore VB, Okada M, Newman PJ, Newman DK. Paxillin family members function as Csk-binding proteins that regulate Lyn activity in human and murine platelets. Biochem. J. 2007; 403:275-81 (PubMed)
- Rose DM. The role of the alpha4 integrin-paxillin interaction in regulating leukocyte trafficking. Exp. Mol. Med. 2006; 38:191-5 (PubMed)
- Sabe H, Onodera Y, Mazaki Y, Hashimoto S. ArfGAP family proteins in cell adhesion, migration and tumor invasion. Curr. Opin. Cell Biol. 2006; 18:558-64 (PubMed)
- Schaller MD. FAK and paxillin: regulators of N-cadherin adhesion and inhibitors of cell migration?. J. Cell Biol. 2004; 166:157-9 (PubMed)
- Spinardi L, Marchisio PC. Podosomes as smart regulators of cellular adhesion. Eur. J. Cell Biol. 2006; 85:191-4 (PubMed)
- Webb DJ, Donais K, Whitmore LA, Thomas SM, Turner CE, Parsons JT, Horwitz AF. FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly. Nat. Cell Biol. 2004; 6:154-61 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| PXN | none | binding partner | Paxillin | Ectopic expression in HEK cells | 10 co-purifying proteins validated | Horwitz | Schroeder et al., Mayhew et al. |
| PXN | none | phosphorylation site | Paxillin | Ectopic expression in HEK cells | 38 phospho-sites in 79.4% coverage of 791 aa | Horwitz | Schroeder et al., Webb et al., Nayal et al. |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
CG18061 Flybase Entrez |
Paxillin |
Mig: 620.0 Non-Mig: 403.2 |
1.53770 | 141605_at |
|
CG18576 Flybase Entrez |
Paxillin |
Mig: 145.6 Non-Mig: 174.2 |
0.83584 | 146489_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG31794 Entrez Gene: 35215 |
Paxillin |
0.444 0.065 |
1.909 | 1.223 |
Actin: 0.651 Area: 1.239 Ratio: 0.525 |
|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
PXN Entrez Gene: 5829 siRNA catalog |
paxillin mRNA: NM_002859 Library: MAR Classification: cytoskeletal Focal Adhesion Related: Yes |
Final bin: Discordant SMARTpool bin: Low Alamar Avg area: 1.79 Avg Alamar: 0.77 Knockdown %: not done |
ERBB2: Impaired BCL2: Low Alamar |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
PXN Entrez Gene: 5829 siRNA catalog |
paxillin mRNA: NM_002859 Library: Control |
 Link |
 Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00220030 | PXN | SPLICE ISOFORM ALPHA OF PAXILLIN. | 14 | 9 | 20 | 13 | 1.43 |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| PXN | mouse | Paxillin - cultured ES cells | - |
Delayed spreading on fibronectin and laminin, reduced tyrosine phosphorylation of Fak. | completed |
-
|
-
|
Wade et al. |
| PXN | mouse | Paxillin - fibroblasts | - |
Abnormal focal adhesions, reduced cell migration, inefficient localization of Fak, defects in cortical cytoskeleton and cell spreading on fibronectin. | completed |
-
|
-
|
Hagel et al. |
| PXN | mouse | Paxillin | conditional expression of FLAG-mCherry-Paxillin | - |
completed - mouse available |
-
|
Ginsberg |
-
|
| PXN | mouse | Paxillin | - |
Embryonic lethality due to abnormal development of mesodermally derived structures such as the heart and somites. | completed |
-
|
-
|
Hagel et al. |
Constructs » activity page
| Ortholog | Species | Targets | Name | Amino Acid Range | Portion | Protocol | Source |
|---|---|---|---|---|---|---|---|
| PXN | human | Paxillin - mut in Histag,protein not in frame with start | pET28-paxillin | 1-557 | Paxillin -full length | - |
Liddington |
| PXN | human | Paxillin - not soluble poor yield | pGEX4T2-paxillin 38-557 | 38-557 | Paxillin (N-terminal truncated) | - |
Liddington |
Biosensors » activity page
| Ortholog | Species | Name | Type | Source | Publications |
|---|---|---|---|---|---|
| PXN | none | paxillin - (Tyr31) | semisynthetic mutants | Imperiali | Vogel and Imperiali |
| PXN | none | paxillin - (peptide) | Environment sensitive fluorophore | Imperiali | Vogel and Imperiali |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Pathways