GRLF1 Orthologs

Aliases: glucocorticoid receptor DNA binding factor 1, GRLF1, GRF-1, P190A, P190-A, KIAA1722, MGC10745, p190RhoGAP

Processes & Diseases

Processes involved: adhesion

Pfam Domain Structure

From GRLF1 (human)

Members of the GRLF1 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB GRLF1 Grlf1 Grlf1 GRLF1 RhoGAPp190 -
Entrez Gene 2909 232906 306400 425730 32743 -
Uniprot GRLF1_HUMAN GRLF1_MOUSE GRLF1_RAT - - -
OMIM 605277 - - - - -
Reagents - - - - - -
Results 5 available - - - 1 available -
References
  1. Gen Y, Yasui K, Zen K, Nakajima T, Tsuji K, Endo M, Mitsuyoshi H, Minami M, Itoh Y, Tanaka S, Taniwaki M, Arii S, Okanoue T, Yoshikawa T. A novel amplification target, ARHGAP5, promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells. Cancer Lett. 2009; 275:27-34 (PubMed)
  2. Hakoshima T, Shimizu T, Maesaki R. Structural basis of the Rho GTPase signaling. J. Biochem. 2003; 134:327-31 (PubMed)
  3. Heckman BM, Chakravarty G, Vargo-Gogola T, Gonzales-Rimbau M, Hadsell DL, Lee AV, Settleman J, Rosen JM. Crosstalk between the p190-B RhoGAP and IGF signaling pathways is required for embryonic mammary bud development. Dev. Biol. 2007; 309:137-49 (PubMed)
  4. Holinstat M, Knezevic N, Broman M, Samarel AM, Malik AB, Mehta D. Suppression of RhoA activity by focal adhesion kinase-induced activation of p190RhoGAP: role in regulation of endothelial permeability. J. Biol. Chem. 2006; 281:2296-305 (PubMed)
  5. Lener T, Burgstaller G, Crimaldi L, Lach S, Gimona M. Matrix-degrading podosomes in smooth muscle cells. Eur. J. Cell Biol. 2006; 85:183-9 (PubMed)
  6. Matheson SF, Hu KQ, Brouns MR, Sordella R, VanderHeide JD, Settleman J. Distinct but overlapping functions for the closely related p190 RhoGAPs in neural development. Dev. Neurosci. 2006; 28:538-50 (PubMed)
Consortium Results & Data
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG32555
Entrez Gene: 32743
RhoGAPp190
Aliases: Rp190RhoGAP
5.222
0.887
0.882 0.965 Actin: 1.102
Area: 1.019
Ratio: 1.081
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
GRLF1
Aliases: GRF-1, P190A, P190-A, p190RhoGAP
Entrez Gene: 2909
siRNA catalog
glucocorticoid receptor DNA binding factor 1
mRNA: NM_024342
Library: MAR
Classification: GTPase regulator
Focal Adhesion Related: Yes
Final bin: Discordant
SMARTpool bin: No change
Avg area: 1.58
Avg Alamar: 0.95
Knockdown %: not done
  GRLF1
Link
GRLF1
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
GRLF1
Aliases: GRF-1, P190A, P190-A, p190RhoGAP
Entrez Gene: 2909
siRNA catalog
glucocorticoid receptor DNA binding factor 1
mRNA: NM_024342
Library: MAR
GRLF1
Link
GRLF1
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00334715 GRLF1 SPLICE ISOFORM 2 OF GLUCOCORTICOID RECEPTOR DNA BINDING FACTOR 1. 6 5 13 11 2.17
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
IPI Locus Description CB Spectra PD Spectra ratio Location
IPI00334715 GRLF1 SPLICE ISOFORM 2 OF GLUCOCORTICOID RECEPTOR DNA BINDING FACTOR 1. 6 13 2.17 nucleus
IPI00334715 GRLF1 SPLICE ISOFORM 2 OF GLUCOCORTICOID RECEPTOR DNA BINDING FACTOR 1. 6 13 2.17 cytoskeleton
Proteomics» Table 3. Quantitation of phosphopeptides identified in Pseudopodium (PD) and Cell Body (CB) fractions
IPI Locus Description peptide pdcb_p pdcb_t ratio Mouse homology Rat homology Zebrafish homology sd Variable sites
IPI00334715 GRLF1 SPLICE ISOFORM 2 OF GLUCOCORTICOID RECEPTOR DNA BINDING FACTOR 1. TSFSVGS*DDELGPIR 6.13 2.17 2.83 +++ +++ ++ 0.01