NCK1 Orthologs

Aliases: NCK tyrosine kinase, NCK adaptor protein 1, melanoma NCK protein, SH2/SH3 adaptor protein NCK-alpha, non-catalytic region of tyrosine kinase, NCK, NCK1, MGC12668, NCKalpha

Pfam Domain Structure

From NCK1 (human)

Members of the NCK1 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB NCK1 Nck1 Nck1 - dock -
Entrez Gene 4690 17973 300955 - 33262 -
Uniprot NCK1_HUMAN - - - - -
OMIM 600508 - - - - -
Reagents - 2 available - - - -
Results 3 available - - - 2 available -
References
  1. Baumgartner M, Sillman AL, Blackwood EM, Srivastava J, Madson N, Schilling JW, Wright JH, Barber DL. The Nck-interacting kinase phosphorylates ERM proteins for formation of lamellipodium by growth factors. Proc. Natl. Acad. Sci. U.S.A. 2006; 103:13391-6 (Full Text)
  2. Blasutig IM, New LA, Thanabalasuriar A, Dayarathna TK, Goudreault M, Quaggin SE, Li SS, Gruenheid S, Jones N, Pawson T. Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization. Mol. Cell. Biol. 2008; 28:2035-46 (Full Text)
  3. Buday L, Wunderlich L, Tamás P. The Nck family of adapter proteins: regulators of actin cytoskeleton. Cell. Signal. 2002; 14:723-31 (PubMed)
  4. Li W, Fan J, Woodley DT. Nck/Dock: an adapter between cell surface receptors and the actin cytoskeleton. Oncogene 2001; 20:6403-17 (PubMed)
  5. Rivera GM, Antoku S, Gelkop S, Shin NY, Hanks SK, Pawson T, Mayer BJ. Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B. Proc. Natl. Acad. Sci. U.S.A. 2006; 103:9536-41 (Full Text)
Consortium Results & Data
Discovery» wt-migratory_cells vs wt-non-migratory_cells
Symbol Description Median Values Fold Change Probe ID
dock
Flybase
Entrez
CMCKB
dreadlocks Mig: 118.4
Non-Mig: 82.1
1.44210 142879_at
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG3727
Entrez Gene: 33262
dreadlocks 10.938
1.029
0.999 1.043 Actin: 1.063
Area: 0.879
Ratio: 1.209
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
NCK1
Aliases: NCK, NCKalpha
Entrez Gene: 4690
siRNA catalog
NCK adaptor protein 1
mRNA: NM_006153
Library: MAR
Classification: adaptor
Final bin: Accelerated - MC
SMARTpool bin: Accelerated
Avg area: 0.27
Avg Alamar: 1.04
Knockdown %: not done
ERBB2: Accelerated
NCK1
Link
NCK1
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
NCK1
Aliases: NCK, NCKalpha
Entrez Gene: 4690
siRNA catalog
NCK adaptor protein 1
mRNA: NM_006153
Library: MAR
NCK1
Link
NCK1
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00028065 NCK1 CYTOPLASMIC PROTEIN NCK1. 1 1 0 0 0.00
Consortium Related Products*
Mice » activity page
Ortholog Species Name Method Phenotype Status Jax Availability Source Publications
NCK1 mouse Nck1 / Nck2 - embryonic fibroblasts
-
Defects in cell motility and organization of the lamellipodial actin network. completed
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-
Bladt et al.
NCK1 mouse Nck1 / Nck2
-
Nck genes have broad and overlapping expression pattern and mice deficient in either Nck1 or Nck2 are viable, whereas inactivation of both resulted in profound defects in mesoderm-derived notochord and embryonic lethality at day 9.5. completed
-
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Bladt et al.

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.