DOCK1 Orthologs

Aliases: dedicator of cyto-kinesis 1, dedicator of cytokinesis 1, DOCK180, DOCK1, RP11-82L9.1, ced5

Processes & Diseases

Processes involved: adhesion

Pfam Domain Structure

From DOCK1 (human)

Members of the DOCK1 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB DOCK1 Dock1 Dock1 - mbc ced-5
Entrez Gene 1793 330662 309081 - 42817 177942
Uniprot DOCK1_HUMAN DOCK1_MOUSE - - - -
OMIM 601403 - - - - -
Reagents 1 available 1 available - - - -
Results 4 available - - - 3 available 1 available
References
  1. Côté JF, Vuori K. Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity. J. Cell. Sci. 2002; 115:4901-13 (PubMed)
  2. Grimsley CM, Kinchen JM, Tosello-Trampont AC, Brugnera E, Haney LB, Lu M, Chen Q, Klingele D, Hengartner MO, Ravichandran KS. Dock180 and ELMO1 proteins cooperate to promote evolutionarily conserved Rac-dependent cell migration. J. Biol. Chem. 2004; 279:6087-97 (PubMed)
  3. Li W, Fan J, Woodley DT. Nck/Dock: an adapter between cell surface receptors and the actin cytoskeleton. Oncogene 2001; 20:6403-17 (PubMed)
  4. Lu M, Kinchen JM, Rossman KL, Grimsley C, Hall M, Sondek J, Hengartner MO, Yajnik V, Ravichandran KS. A Steric-inhibition model for regulation of nucleotide exchange via the Dock180 family of GEFs. Curr. Biol. 2005; 15:371-7 (PubMed)
  5. Reif K, Cyster J. The CDM protein DOCK2 in lymphocyte migration. Trends Cell Biol. 2002; 12:368-73 (PubMed)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
DOCK1 none structure

PIP3 binding DHR1 domain of DOCK180

X-Ray crystallography

completed Liddington
-
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
DOCK1
Aliases: ced5, DOCK180
Entrez Gene: 1793
siRNA catalog
dedicator of cytokinesis 1
mRNA: NM_001380
Library: MAR
Classification: GTPase regulator
Focal Adhesion Related: Yes
Final bin: No Phenotype
SMARTpool bin: No change
Avg area: 1.25
Avg Alamar: 0.93
Knockdown %: not done
  DOCK1
Link
DOCK1
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
DOCK1
Aliases: ced5, DOCK180
Entrez Gene: 1793
siRNA catalog
dedicator of cytokinesis 1
mRNA: NM_001380
Library: MAR
DOCK1
Link
DOCK1
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00015286 DOCK1 DEDICATOR OF CYTOKINESIS PROTEIN 1. 4 4 7 5 1.75
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
IPI Locus Description CB Spectra PD Spectra ratio Location
IPI00015286 DOCK1 DEDICATOR OF CYTOKINESIS PROTEIN 1. 4 7 1.75 cytoskeleton
Discovery» Drosophila Migration Genes
Gene Name Function Cell
Type
Phenotypes Reagents/
Alleles
Reference
mbc
Entrez: CG10379
Flybase: FBgn0015513
myoblast city Rac exchange factor, DOCK180 border cells Loss of function: incomplete border cell migration
Autonomous: autonomous
Gain of function: not reported

Comments: enhances reduction of elmo
mbcC2, mbcD11.2, FRT, UAS-mbc RNAi Duchek 2001; Bianco 2007; Geisbrecht 2008
dorsal closure Loss of function: dorsal closure defects (defects in cytoskeletal organization in the migrating epidermis) Df(3R)mbc-30, Df(3R)mbc-15, mbcS4 Erickson, 1997
embryonic hemocyte Loss of function: no phenotype
Gain of function: ND

Comments: Deficiency that removes mbc has phenotype
Df(3R)mbc-30, Df(3R)mbc-15, reported nulls: mbcC1, mbcD11.2 Paladi 2004
Discovery» wt-migratory_cells vs wt-non-migratory_cells
Symbol Description Median Values Fold Change Probe ID
mbc
Flybase
Entrez
CMCKB
myoblast city Mig: 511.3
Non-Mig: 325.2
1.57230 143808_at
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG10379
Entrez Gene: 42817
myoblast city 11.250
1.449
0.837 0.93 Actin: 0.962
Area: 0.849
Ratio: 1.133
Discovery» C. elegans genes affecting migration
Gene Description/Function Parental P0 defect Distribution1
(percent)
Progeny F1 defect Distribution2
(percent)
Express DTC Express BWM
Gene: ced‑5 (C02F4.1)
Gene Pair ID
CMKB
Entrez Gene
CEll Death abnormality
AhringerAutoClass: Cell Architecture
Function: Cell Architecture
InterPro: *Small GTPase regulatory/interacting protein
0.38 (N=98)
0.28 – 0.48
Type 1: 16
Type 2: 27
Type 3: 57
0.7 (N=86)
0.59 – 0.79
Type 1: 7
Type 2: 46
Type 3: 47
   
Consortium Related Products*
Mice » activity page
Ortholog Species Name Method Phenotype Status Jax Availability Source Publications
DOCK1 mouse DOCK 180 Targeted - floxed and non-floxed
-
completed, publication in progress
-
Burds Laurin et al.
Constructs » activity page
Ortholog Species Targets Name Amino Acid Range Portion Protocol Source
DOCK1 human DOCK180 pGEX-6P2 154-256 DOCK180 DHR1 domain available Liddington

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.

Pathways

How many degrees of interaction?

Update
Legend: Legend for CMKB pathways