RAC1 Orthologs

Aliases: rho family, small GTP binding protein Rac1, Ras-like protein TC25, Cell migration-inducing gene 5 protein, ras-related C3 botulinum toxin substrate 1, migration-inducing gene 5, migration-inducing protein 5, ras-related C3 botulinum toxin substrate 1 isoform Rac1b, TC-25, RAC1, MGC111543, MIG5, p21-Rac1

Processes & Diseases

Processes involved: polarity

Pfam Domain Structure

From RAC1 (human)

Members of the RAC1 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB RAC1 Rac1 Rac1 RAC1 Rac2 ced-10
Entrez Gene 5879 19353 363875 395871 38831 177111
Uniprot RAC1_HUMAN RAC1_MOUSE RAC1_RAT RAC1_BOVIN RAC2_DROME RAC1_CAEEL
OMIM 602048 - - - - -
Reagents - - - - - -
Results 3 available - - - 3 available 1 available
References
  1. Chan JR. Myelination: all about Rac 'n' roll. J. Cell Biol. 2007; 177:953-5 (Full Text)
  2. Heasman SJ, Ridley AJ. Mammalian Rho GTPases: new insights into their functions from in vivo studies. Nat. Rev. Mol. Cell Biol. 2008; 9:690-701 (PubMed)
  3. Nikolova E, Mitev V, Zhelev N, Deroanne CF, Poumay Y. The small Rho GTPase Rac1 controls normal human dermal fibroblasts proliferation with phosphorylation of the oncoprotein c-myc. Biochem. Biophys. Res. Commun. 2007; 359:834-9 (PubMed)
  4. Palamidessi A, Frittoli E, Garré M, Faretta M, Mione M, Testa I, Diaspro A, Lanzetti L, Scita G, Di Fiore PP. Endocytic trafficking of Rac is required for the spatial restriction of signaling in cell migration. Cell 2008; 134:135-47 (PubMed)
  5. Ridley AJ. Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking. Trends Cell Biol. 2006; 16:522-9 (PubMed)
  6. Ridley AJ, Paterson HF, Johnston CL, Diekmann D, Hall A. The small GTP-binding protein rac regulates growth factor-induced membrane ruffling. Cell 1992; 70:401-10 (PubMed)
  7. Rose DM, Alon R, Ginsberg MH. Integrin modulation and signaling in leukocyte adhesion and migration. Immunol. Rev. 2007; 218:126-34 (PubMed)
  8. Rottner K, Hall A, Small JV. Interplay between Rac and Rho in the control of substrate contact dynamics. Curr. Biol. 1999; 9:640-8 (PubMed)
  9. Sander EE, ten Klooster JP, van Delft S, van der Kammen RA, Collard JG. Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior. J. Cell Biol. 1999; 147:1009-22 (Full Text)
  10. Watabe-Uchida M, Govek EE, Van Aelst L. Regulators of Rho GTPases in neuronal development. J. Neurosci. 2006; 26:10633-5 (PubMed)
  11. Wittmann T, Bokoch GM, Waterman-Storer CM. Regulation of microtubule destabilizing activity of Op18/stathmin downstream of Rac1. J. Biol. Chem. 2004; 279:6196-203 (PubMed)
  12. Yang N, Higuchi O, Ohashi K, Nagata K, Wada A, Kangawa K, Nishida E, Mizuno K. Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization. Nature 1998; 393:809-12 (PubMed)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
RAC1 none binding partner Rac1 Ectopic expression in HEK cells data available Horwitz
-
Discovery» C. elegans genes affecting migration
Gene Description/Function Parental P0 defect Distribution1
(percent)
Progeny F1 defect Distribution2
(percent)
Express DTC Express BWM
Gene: ced‑10 (C09G12.8)
Gene Pair ID
CMKB
Entrez Gene
CEll Death abnormality
AhringerAutoClass: Signaling
Function: Signaling
InterPro: GTPase
0.58 (N=60)
0.45 – 0.71
Type 1: 7
Type 2: 27
Type 3: 67
0.65 (N=46)
0.5 – 0.79
Type 1: 8
Type 2: 31
Type 3: 58
Yes
Yes
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
RAC1
Aliases: MIG5, TC-25, p21-Rac1
Entrez Gene: 5879
siRNA catalog
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
mRNA: NM_018890
Library: MAR
Classification: GTPase
Focal Adhesion Related: Yes
Final bin: Impaired - MC
SMARTpool bin: Impaired
Avg area: 2.62
Avg Alamar: 0.83
Knockdown %: not done
ERBB2: Impaired
RAC1
Link
RAC1
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
RAC1
Aliases: MIG5, TC-25, p21-Rac1
Entrez Gene: 5879
siRNA catalog
ras-related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
mRNA: NM_018890
Library: MAR
RAC1
Link
RAC1
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00010271 RAC1 SPLICE ISOFORM A OF RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 1. 31 11 66 14 2.13
Discovery» Drosophila Migration Genes
Gene Name Function Cell
Type
Phenotypes Reagents/
Alleles
Reference
Rac2
Entrez: CG8556
Flybase: FBgn0014011
Rac2 Rho GTPase activity dorsal closure Loss of function: no phenotype

Comments: mild dorsal closure phneotype in combiantion with rac1; strong pheotpe in comination with rac1 and rac3
rac2delta Hakeda-Suzuki, 2002
embryonic hemocyte Loss of function: rac2D no phenotype, In MZ hypomorphic allele + rac2D embryo hemocytes fail to migrate into posterior vnc

Comments: ND
null: rac2D Paladi 2004
embryonic hemocyte to wound Loss of function: rac1D , rac2D, MtlD triple mutant strongly reduced recruitment to wounds, reduced lamellipodia

Comments: ND
null: rac2D Stramer 2005
embryonic mesoderm Loss of function: Maternal and zygotic Rac1,Rac2 double mutants affect mesoderm spreading
Autonomous: autonomous
Rac2∆ van Impel et al., 2009
Discovery» wt-migratory_cells vs wt-non-migratory_cells
Symbol Description Median Values Fold Change Probe ID
Rac2
Flybase
Entrez
CMCKB
CG8556 gene product from transcript CG8556-RA Mig: 607.4
Non-Mig: 385.0
1.57770 153313_at
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG8556
Entrez Gene: 38831
Rac2
Aliases: Rac
8.250
1.689
1.309 1.179 Actin: 1.257
Area: 0.409
Ratio: 3.073
Consortium Related Products*
Biosensors » activity page
Ortholog Species Name Type Source Publications
RAC1 none Cdc42 / Rac Intramolecular FRET between FPs - EBFP-PBD of PAK-EGFP
-
Graham et al.
RAC1 none Rac1 Intermolecular FRET between FP and fluorescent dye - GFP-Rac1; Alexa546-PBD from PAK (FLAIR)
-
Kraynov et al.
RAC1 none Rac1 / Cdc42 Intramolecular FRET between FPs - YFP-PBD (PAK)-Rac1/Cdc42-CFP-Farnesyl moiety (Raichu)
-
Itoh et al.
RAC1 none rac - (Rac1) three-segment ligation and two-segment - Make Rac by in vitro semi-synthesis for caged Rho GTPase biosensor Imperiali
-
RAC1 none rac - (Rac1) three-segment ligation - Make Rac amino acids for caged Rho GTPase biosensor Imperiali
-
RAC1 none rac FRET - dual chain Hahn Chamberlain et al., Kraynov et al., Gardiner et al.
RAC1 none rac Generically encoded caged Rac Hahn Wang et al.
RAC1 none rac - (Rac1) FRET - single chain Hahn
-

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.