CAPN2 Orthologs
Aliases: Calcium-activated neutral proteinase 2, calpain, large polypeptide L2, calpain 2, large subunit, M-calpain, calpain M-type, millimolar-calpain, calpain 2, large [catalytic] subunit, calcium-activated neutral proteinase, CAPN2, FLJ39928, CANP 2, mCANP, CANP2, CANPL2, CANPml
Pfam Domain Structure
From CAPN2 (human) - phosphorylation sites added via direct mapping
Members of the CAPN2 ortholog set
| Human | Mouse | Rat | Chicken | Fly | Worm | |
| CMKB | CAPN2 | Capn2 | Capn2 | CAPN2 | CalpB | - |
| Entrez Gene | 824 | 12334 | 29154 | 395963 | 39165 | - |
| Uniprot | CAN2_HUMAN | CAN2_MOUSE | CAN2_RAT | CAN2_CHICK | CANB_DROME | - |
| OMIM | 114230 | - | - | - | - | - |
| Reagents | - | 1 available | - | - | - | - |
| Results | 3 available | - | - | - | 2 available | - |
References
- Carragher NO, Walker SM, Scott Carragher LA, Harris F, Sawyer TK, Brunton VG, Ozanne BW, Frame MC. Calpain 2 and Src dependence distinguishes mesenchymal and amoeboid modes of tumour cell invasion: a link to integrin function. Oncogene 2006; 25:5726-40 (PubMed)
- Chan KT, Bennin DA, Huttenlocher A. Regulation of adhesion dynamics by calpain-mediated proteolysis of focal adhesion kinase (FAK). J. Biol. Chem. 2010; 285:11418-26 (PubMed)
- Fernández-Montalván A, Assfalg-Machleidt I, Pfeiler D, Fritz H, Jochum M, Machleidt W. Mu-calpain binds to lipid bilayers via the exposed hydrophobic surface of its Ca2+-activated conformation. Biol. Chem. 2006; 387:617-27 (PubMed)
- Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, Huttenlocher A. Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat. Cell Biol. 2004; 6:977-83 (PubMed)
- Goll DE, Thompson VF, Li H, Wei W, Cong J. The calpain system. Physiol. Rev. 2003; 83:731-801 (PubMed)
- Huttenlocher A, Palecek SP, Lu Q, Zhang W, Mellgren RL, Lauffenburger DA, Ginsberg MH, Horwitz AF. Regulation of cell migration by the calcium-dependent protease calpain. J. Biol. Chem. 1997; 272:32719-22 (PubMed)
- Su LT, Agapito MA, Li M, Simonson WT, Huttenlocher A, Habas R, Yue L, Runnels LW. TRPM7 regulates cell adhesion by controlling the calcium-dependent protease calpain. J. Biol. Chem. 2006; 281:11260-70 (PubMed)
- Wu M, Yu Z, Fan J, Caron A, Whiteway M, Shen SH. Functional dissection of human protease mu-calpain in cell migration using RNAi. FEBS Lett. 2006; 580:3246-56 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| CAPN2 | none | binding partner | Calpain 2 | Ectopic expression in HEK cells | Proteins co-purifying with expressed FLAG-tagged calpain | Horwitz |
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| CAPN2 | none | phosphorylation site | Calpain 2 | Ectopic expression in HEK cells | 4 phospho-sites in 88.1% coverage of 708 aa | Parsons |
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Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
CAPN2 Aliases: mCANP, CANPL2, CANPml Entrez Gene: 824 siRNA catalog |
calpain 2, (m/II) large subunit mRNA: NM_001748 Library: MAR Classification: protease |
Final bin: Discordant SMARTpool bin: Low Alamar Avg area: 2.94 Avg Alamar: 0.68 Knockdown %: not done |
ERBB2: Impaired BCL2: Low Alamar |
%20large%20subunit&lib=MAR&fbin=Discordant) Link |
%20large%20subunit&lib=MAR&fbin=Discordant) Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
CAPN2 Aliases: mCANP, CANPL2, CANPml Entrez Gene: 824 siRNA catalog |
calpain 2, (m/II) large subunit mRNA: NM_001748 Library: MAR |
%20large%20subunit&lib=MAR) Link |
%20large%20subunit&lib=MAR) Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00289758 | CAPN2 | CALPAIN 2, LARGE [CATALYTIC] SUBUNIT PRECURSOR. | 41 | 15 | 41 | 20 | 1.00 |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
CalpB Flybase Entrez CMCKB |
Calpain-B |
Mig: 307.8 Non-Mig: 448.9 |
0.68568 | 151648_at |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| CAPN2 | mouse | Calpain II (m-calpain) (Capn2) | - |
None, though elimination of the Capn4 gene (see below) destroys the CalpainII complex. | completed |
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Arthur et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.