PTK2 Orthologs

Aliases: focal adhesion kinase 1, Protein-tyrosine kinase 2, PTK2 protein tyrosine kinase 2, FAK, FADK 1, PTK2, FADK, FAK1, pp125FAK

Processes & Diseases

Processes involved: adhesion

Pfam Domain Structure

From PTK2 (chicken) - phosphorylation sites added via direct mapping

Members of the PTK2 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB PTK2 Ptk2 Ptk2 PTK2 Fak56D kin-32
Entrez Gene 5747 14083 25614 396416 37233 172135
Uniprot FAK1_HUMAN FAK1_MOUSE FAK1_RAT FAK1_CHICK - -
OMIM 600758 - - - - -
Reagents 2 available 4 available - - - -
Results 4 available 1 available - - 1 available -
References
  1. Cohen LA, Guan JL. Mechanisms of focal adhesion kinase regulation. Curr Cancer Drug Targets 2005; 5:629-43 (PubMed)
  2. Cox BD, Natarajan M, Stettner MR, Gladson CL. New concepts regarding focal adhesion kinase promotion of cell migration and proliferation. J. Cell. Biochem. 2006; 99:35-52 (PubMed)
  3. Lim ST, Mikolon D, Stupack DG, Schlaepfer DD. FERM control of FAK function: implications for cancer therapy. Cell Cycle 2008; 7:2306-14 (Full Text)
  4. Mitra SK, Schlaepfer DD. Integrin-regulated FAK-Src signaling in normal and cancer cells. Curr. Opin. Cell Biol. 2006; 18:516-23 (PubMed)
  5. Mon NN, Ito S, Senga T, Hamaguchi M. FAK signaling in neoplastic disorders: a linkage between inflammation and cancer. Ann. N. Y. Acad. Sci. 2006; 1086:199-212 (PubMed)
  6. Parsons JT. Focal adhesion kinase: the first ten years. J. Cell. Sci. 2003; 116:1409-16 (PubMed)
  7. Schaller MD. FAK and paxillin: regulators of N-cadherin adhesion and inhibitors of cell migration?. J. Cell Biol. 2004; 166:157-9 (Full Text)
  8. Tomar A, Schlaepfer DD. Focal adhesion kinase: switching between GAPs and GEFs in the regulation of cell motility. Curr. Opin. Cell Biol. 2009; 21:676-83 (Full Text)
  9. Webb DJ, Donais K, Whitmore LA, Thomas SM, Turner CE, Parsons JT, Horwitz AF. FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly. Nat. Cell Biol. 2004; 6:154-61 (PubMed)
  10. Wozniak MA, Modzelewska K, Kwong L, Keely PJ. Focal adhesion regulation of cell behavior. Biochim. Biophys. Acta 2004; 1692:103-19 (PubMed)
  11. Xie Z, Tsai LH. Cdk5 phosphorylation of FAK regulates centrosome-associated miocrotubules and neuronal migration. Cell Cycle 2004; 3:108-10 (PubMed)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
PTK2 none phosphorylation site FAK Ectopic expression in HEK cells 18 phospho-sites in 80.2% coverage of 1045 aa Parsons Grigera et al.
PTK2 none structure

Paxillin (1-328)-FAT domain of FAK

NMR

completed Campbell
-
PTK2 none structure

Full-length FAK

X-Ray crystallography

completed Liddington
-
PTK2 none structure FAT domain of Focal Adhesion Kinase X-Ray crystallography, NMR data available Liddington Hayashi et al.
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG10023
Entrez Gene: 37233
Focal Adhesion Kinase 7.688
1.202
1.041 1.061 Actin: 0.978
Area: 0.91
Ratio: 1.075
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
PTK2
Aliases: FAK, FADK, FAK1, pp125FAK
Entrez Gene: 5747
siRNA catalog
PTK2 protein tyrosine kinase 2
mRNA: NM_005607
Library: Kinase
Classification: Ser/Thr Kinase
Focal Adhesion Related: Yes
Final bin: Discordant
SMARTpool bin: No change
Avg area: 1.28
Avg Alamar: 0.83
Knockdown %: 70-80
  PTK2
Link
PTK2
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
PTK2
Aliases: FAK, FADK, FAK1, pp125FAK
Entrez Gene: 5747
siRNA catalog
PTK2 protein tyrosine kinase 2
mRNA: NM_005607
Library: Kinase
PTK2
Link
PTK2
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00012885 PTK2 SPLICE ISOFORM 1 OF FOCAL ADHESION KINASE 1. 4 4 14 8 3.50
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
IPI Locus Description CB Spectra PD Spectra ratio Location
IPI00012885 PTK2 SPLICE ISOFORM 1 OF FOCAL ADHESION KINASE 1. 4 14 3.5 cytoskeleton
Discovery» MTLn3 invasive data
Gene mRNA Description Expression Ratio
Ptk2
Entrez Gene: 14083
mRNA: AW536852
UniGene: Mm.254494
PTK2 protein tyrosine kinase 2 2.2
Consortium Related Products*
Mice » activity page
Ortholog Species Name Method Phenotype Status Jax Availability Source Publications
PTK2 mouse FAK / Cas - double knockout in embryonic fibroblasts
-
Defects in ephrinA1-induced cell spreading. completed
-
-
Carter et al.
PTK2 mouse Fak
-
Death at E8.5 with impaired blood vessel development. completed
-
-
Ilic et al.
PTK2 mouse FAK - embryonic fibroblasts
-
Reduced migration rate and an increase in the number and size of peripherally localized adhesions. completed
-
-
Ilić et al.
PTK2 mouse FAK
-
Elevated levels of Pyk2. completed
-
-
Sieg et al.
Constructs » activity page
Ortholog Species Targets Name Amino Acid Range Portion Protocol Source
PTK2 human FAK - soluble expression FAK-baculovirus 1-1052 FAK-full length
-
Liddington
PTK2 human FAK pT7-7-FAK 1-1052 FAK-full length available Liddington
Biosensors » activity page
Ortholog Species Name Type Source Publications
PTK2 none FAK Intermolecular FRET between FPs - CFP-FAK; dSH2 (Src)-YFP
-
Cai et al.
PTK2 none FAK Intramolecular FRET between FPs - Insertion of a FRET pair in FAK
-
Cai et al.
PTK2 none FAK FRET - single chain Hahn Cai et al.
Imaging Probes » activity page
Ortholog Species Name Type Status Source Publications
PTK2 none Photoactivation Fak/PTK2 - (caged phosphoTyr (Y*) - cPYFAK, probe sequence; VSETDDY*AEIIDEEDT-NH2) completed Imperiali, Jacobson Humphrey et al.

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.

Pathways

How many degrees of interaction?

Update
Legend: Legend for CMKB pathways