ACTN1 Orthologs

Aliases: actinin, alpha 1, alpha-actinin 1, actinin 1 smooth muscle, F-actin cross-linking protein, ACTN1, FLJ40884, FLJ54432

Pfam Domain Structure

From ACTN1 (human) - phosphorylation sites added via direct mapping

Members of the ACTN1 ortholog set
Human Mouse Rat Chicken Fly Worm
CMKB ACTN1 Actn1 Actn1 ACTN1 Actn atn-1
Entrez Gene 87 109711 81634 373918 31166 179709
Uniprot ACTN1_HUMAN ACTN1_MOUSE ACTN1_RAT ACTN1_CHICK ACTN_DROME -
OMIM 102575 - - - - -
Reagents 2 available - - - - -
Results 4 available - - - 3 available -
Ortholog Summary

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene [Entrez Gene].

References
  1. Cabello N, Remelli R, Canela L, Soriguera A, Mallol J, Canela EI, Robbins MJ, Lluis C, Franco R, McIlhinney RA, Ciruela F. Actin-binding protein alpha-actinin-1 interacts with the metabotropic glutamate receptor type 5b and modulates the cell surface expression and function of the receptor. J. Biol. Chem. 2007; 282:12143-53 (PubMed)
  2. Choi CK, Vicente-Manzanares M, Zareno J, Whitmore LA, Mogilner A, Horwitz AR. Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nat. Cell Biol. 2008; 10:1039-50 (Full Text)
  3. Courson DS, Rock RS. Actin cross-link assembly and disassembly mechanics for alpha-Actinin and fascin. J. Biol. Chem. 2010; 285:26350-7 (Full Text)
  4. Izaguirre G, Aguirre L, Hu YP, Lee HY, Schlaepfer DD, Aneskievich BJ, Haimovich B. The cytoskeletal/non-muscle isoform of alpha-actinin is phosphorylated on its actin-binding domain by the focal adhesion kinase. J. Biol. Chem. 2001; 276:28676-85 (PubMed)
  5. Lazarides E, Burridge K. Alpha-actinin: immunofluorescent localization of a muscle structural protein in nonmuscle cells. Cell 1975; 6:289-98 (PubMed)
  6. Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol. Rev. 2008; 88:489-513 (PubMed)
  7. Otey CA, Pavalko FM, Burridge K. An interaction between alpha-actinin and the beta 1 integrin subunit in vitro. J. Cell Biol. 1990; 111:721-9 (Full Text)
  8. Wahlström G, Norokorpi HL, Heino TI. Drosophila alpha-actinin in ovarian follicle cells is regulated by EGFR and Dpp signalling and required for cytoskeletal remodelling. Mech. Dev. 2006; 123:801-18 (PubMed)
  9. Zhang W, Gunst SJ. Dynamic association between alpha-actinin and beta-integrin regulates contraction of canine tracheal smooth muscle. J. Physiol. (Lond.) 2006; 572:659-76 (Full Text)
  10. Zhang Z, Lin SY, Neel BG, Haimovich B. Phosphorylated alpha-actinin and protein-tyrosine phosphatase 1B coregulate the disassembly of the focal adhesion kinase x Src complex and promote cell migration. J. Biol. Chem. 2006; 281:1746-54 (PubMed)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
ACTN1 none structure Alpha-actinin cross-links 2D crystallization on lipid monolayers completed Taylor Hampton et al.
ACTN1 none structure

Organization of zyxin and alpha-actinin in focal adhesions and stress fibers

Correlative light microscopy-electron microscopy

completed Geiger
-
ACTN1 none structure

Alpha-actinin1 (plus calcium)

2D crystallization on lipid monolayers

completed Taylor
-
ACTN1 none structure

Organization of zyxin and alpha-actinin in focal adhesions and stress fibers

Correlative light microscopy-electron microscopy

completed Geiger
-
ACTN1 none structure

Vinculin N-terminal domain complex with alpha-actinin1 domain 3

X-Ray crystallography

completed Liddington
-
ACTN1 none structure

Alpha-actinin1 / F-actin complexes

Electron Tomography

completed Taylor Collins et al.
ACTN1 none structure

Alpha-actinin1 (in EGTA)

2D crystallization on lipid monolayers

completed Taylor
-
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
ACTN1
Entrez Gene: 87
siRNA catalog
actinin, alpha 1
mRNA: NM_001102
Library: MAR
Classification: cytoskeletal
Focal Adhesion Related: Yes
Final bin: Discordant
SMARTpool bin: No change
Avg area: 1.79
Avg Alamar: 0.96
Knockdown %: not done
  ACTN1
Link
ACTN1
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
ACTN1
Entrez Gene: 87
siRNA catalog
actinin, alpha 1
mRNA: NM_001102
Library: MAR
ACTN1
Link
ACTN1
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00013508 ACTN1 ALPHA-ACTININ 1. 305 61 549 77 1.80
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
IPI Locus Description CB Spectra PD Spectra ratio Location
IPI00013508 ACTN1 ALPHA-ACTININ 1. 305 549 1.8 cytoskeleton
Discovery» Genes encoding cytoskeleton-associated proteins enriched in migratory cells
Gene Description Nearest mammalian (Human) homolog Fold Change
Actn
GeneID:31166
Entrez
Flybase
alpha-actinin
Proposed Function: F-actin crosslinking Links talin and F-actin
Conserved Protein Domains: CH, EF hand, Spectrin repeats
ACTN1
GeneID: 87
CMKB Orthologs
1.38
Discovery» wt-migratory_cells vs wt-non-migratory_cells
Symbol Description Median Values Fold Change Probe ID
Actn
Flybase
Entrez
CMCKB
alpha actinin Mig: 1096.2
Non-Mig: 739.5
1.48240 154816_at
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG4376
Entrez Gene: 31166
alpha actinin
Aliases: Alpha-actinin; alpha-actinin; flightless A; alpha-Actinin; lethal(1)2cb; a-actinin; flightless-A
13.188
1.081
1.012 0.973 Actin: 0.886
Area: 1.117
Ratio: 0.793
Consortium Related Products*
Constructs » activity page
Ortholog Species Targets Name Amino Acid Range Portion Protocol Source
ACTN1 human Alpha Actinin - soluble pET15b-aActinin-SR 269-444 Alpha actinin - spectrin repeats
-
Liddington
ACTN1 human Alpha Actinin - soluble pET15b-aActinin 1-892 Alpha actinin - full length available Liddington

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.

Pathways

How many degrees of interaction?

Update
Legend: Legend for CMKB pathways