TLN1 human: talin 1
Aliases: talin 1, TLN, TLN1, ILWEQ, KIAA1027
Pfam Domain Structure
From TLN1 (human) - phosphorylation sites added via direct mapping
Protein Overview
| Official Gene Name | TLN1 (H. sapiens) |
| RefSeq Protein Name | talin 1 |
| UniProt Gene Symbol | TLN1_HUMAN |
| UniProt Name | Talin-1 |
| Entrez Gene Summary | This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq] |
References
- Abrams CS. Intracellular signaling in platelets. Curr. Opin. Hematol. 2005; 12:401-5 (PubMed)
- Burridge K, Mangeat P. An interaction between vinculin and talin. Nature 1984; 308:744-6 (PubMed)
- Calderwood DA. Talin controls integrin activation. Biochem. Soc. Trans. 2004; 32:434-7 (PubMed)
- Campbell ID, Ginsberg MH. The talin-tail interaction places integrin activation on FERM ground. Trends Biochem. Sci. 2004; 29:429-35 (PubMed)
- Cram EJ, Schwarzbauer JE. The talin wags the dog: new insights into integrin activation. Trends Cell Biol. 2004; 14:55-7 (PubMed)
- Critchley DR. Biochemical and structural properties of the integrin-associated cytoskeletal protein talin. Annu Rev Biophys 2009; 38:235-54 (PubMed)
- Critchley DR. Cytoskeletal proteins talin and vinculin in integrin-mediated adhesion. Biochem. Soc. Trans. 2004; 32:831-6 (PubMed)
- Critchley DR. Genetic, biochemical and structural approaches to talin function. Biochem. Soc. Trans. 2005; 33:1308-12 (PubMed)
- del Rio A, Perez-Jimenez R, Liu R, Roca-Cusachs P, Fernandez JM, Sheetz MP. Stretching single talin rod molecules activates vinculin binding. Science 2009; 323:638-41 (PubMed)
- Franco SJ, Rodgers MA, Perrin BJ, Han J, Bennin DA, Critchley DR, Huttenlocher A. Calpain-mediated proteolysis of talin regulates adhesion dynamics. Nat. Cell Biol. 2004; 6:977-83 (PubMed)
- Hemmings L, Rees DJ, Ohanian V, Bolton SJ, Gilmore AP, Patel B, Priddle H, Trevithick JE, Hynes RO, Critchley DR. Talin contains three actin-binding sites each of which is adjacent to a vinculin-binding site. J. Cell. Sci. 1996; 109 ( Pt 11):2715-26 (PubMed)
- Horwitz A, Duggan K, Buck C, Beckerle MC, Burridge K. Interaction of plasma membrane fibronectin receptor with talin--a transmembrane linkage. Nature 1986; 320:531-3 (PubMed)
- Le Clainche C, Carlier MF. Regulation of actin assembly associated with protrusion and adhesion in cell migration. Physiol. Rev. 2008; 88:489-513 (PubMed)
- Lin J, Miller MJ, Shaw AS. The c-SMAC: sorting it all out (or in). J. Cell Biol. 2005; 170:177-82 (PubMed)
- Moes M, Rodius S, Coleman SJ, Monkley SJ, Goormaghtigh E, Tremuth L, Kox C, van der Holst PP, Critchley DR, Kieffer N. The integrin binding site 2 (IBS2) in the talin rod domain is essential for linking integrin beta subunits to the cytoskeleton. J. Biol. Chem. 2007; 282:17280-8 (PubMed)
- Nayal A, Webb DJ, Horwitz AF. Talin: an emerging focal point of adhesion dynamics. Curr. Opin. Cell Biol. 2004; 16:94-8 (PubMed)
- Ratnikov BI, Partridge AW, Ginsberg MH. Integrin activation by talin. J. Thromb. Haemost. 2005; 3:1783-90 (PubMed)
- Watanabe N, Bodin L, Pandey M, Krause M, Coughlin S, Boussiotis VA, Ginsberg MH, Shattil SJ. Mechanisms and consequences of agonist-induced talin recruitment to platelet integrin alphaIIbbeta3. J. Cell Biol. 2008; 181:1211-22 (PubMed)
Consortium Results & Data
Results & Data
| Ortholog | Species | Type | Description | Method | Status | Source | Publications |
|---|---|---|---|---|---|---|---|
| TLN1 | none | phosphorylation site | Talin | Endogenous from human platelet | 30 phospho-sites in 95.1% coverage of 2540 aa | Ginsberg | Ratnikov et al. |
| TLN1 | none | structure | Talin (C-terminal Actin-binding domain) | EM, SAXS, docking and helical reconstruction | completed | Hanein | Gingras et al. |
| TLN1 | none | structure | Talin (Ferm-domain) and Actin | EM and helical reconstruction | completed | Hanein | Lee et al. |
| TLN1 | none | structure | Talin -beta3 integrin | X-Ray crystallography, NMR | data available | Liddington | García-Alvarez et al., Wegener et al. |
| TLN1 | none | structure | Layilin-talin (196-400) | X-Ray crystallography, NMR | completed | Liddington | Wegener et al. |
| TLN1 | none | structure | PIPKIg -talin (196-400) | X-Ray crystallography, NMR | completed | Liddington | de Pereda et al. |
Discovery» MTLn3 invasive data
| Gene | mRNA | Description | Expression Ratio |
|
Tln1 Entrez Gene: 21894 |
mRNA: AA208883 UniGene: Mm.208601 |
Talin 1 | 0.3 |
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
TLN1 Aliases: TLN, ILWEQ Entrez Gene: 7094 siRNA catalog |
talin 1 mRNA: NM_006289 Library: MAR Classification: cytoskeletal Focal Adhesion Related: Yes |
Final bin: Impaired - HC SMARTpool bin: Impaired Avg area: 3.61 Avg Alamar: 0.91 Knockdown %: not done |
ERBB2: Impaired |
 Link |
 Link |
Impaired E Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
TLN1 Aliases: TLN, ILWEQ Entrez Gene: 7094 siRNA catalog |
talin 1 mRNA: NM_006289 Library: MAR |
 Link |
 Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00298994 | TLN1 | TALIN 1. | 137 | 49 | 272 | 78 | 1.99 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00298994 | TLN1 | TALIN 1. | 137 | 272 | 1.99 | cytoskeleton |
| IPI00298994 | TLN1 | TALIN 1. | 137 | 272 | 1.99 | cytosol |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| TLN1 | mouse | Talin 1 | - |
Embryonic lethality at E8.5-9.5 with disorganization of embryos at gastrulation. | completed |
-
|
-
|
Monkley et al. |
| TLN1 | mouse | Talin - trophoblast cells | - |
Reduced spreading on fibronectin and laminin. | completed |
-
|
-
|
Monkley et al. |
Constructs » activity page
| Ortholog | Species | Targets | Name | Amino Acid Range | Portion | Protocol | Source |
|---|---|---|---|---|---|---|---|
| TLN1 | human | Talin / PIPKIg - soluble | pET30a-talin | 1-2541 | Talin-full length, S429D gain-of-function mutant | - |
Liddington |
| TLN1 | human | Talin / PIPKIg | pET30b-talin rod | 397-2541 | Talin rod | available | Liddington |
| TLN1 | human | Talin / PIPKIg - soluble | pET30a-talin | 1-2541 | Full length Talin | available | Liddington |
| TLN1 | human | Talin / PIPKIg | pET151-DTOPO talin 196-889 | 196-889 | Talin FERM F2F3 + part of rod | available |
-
|
| TLN1 | human | Talin / PIPKIg - soluble | pET30a-talin | 1-2541 | Talin-full length, S446D gain-of-function mutant | - |
Liddington |
| TLN1 | human | Talin / PIPKIg - soluble | pET30a-talin | 1-2541 | Talin-full length, S429D/S446D double gain-of-function mutant | - |
Liddington |
| TLN1 | human | Talin / PIPKIg | pGEX-4T-2 | 1984-2344 | Talin VBS3 | - |
-
|
| TLN1 | human | Talin / PIPKIg | pET-29+ | Talin Fragment G (L2094A/I12095A mutant) | - |
-
|
|
| TLN1 | human | Talin / PIPKIg | pGEX-4T-2 | 2071-2135 | Talin's actin-binding domain | - |
-
|
| TLN1 | mouse | Layilin / Talin | pET15b-layilin | 207-400 | Layilin tail+talin F2+F3 fusion protein | - |
Liddington |
| TLN1 | mouse | Talin / PIPKIg - soluble/great yield | pET15b-talin 482-636 | 482-636 | Talin's Vinculin-binding domain (VBS1; 4-helix bundle) | available | Liddington |
| TLN1 | mouse | Talin / PIPKIg - soluble/great yield | pET15b-talin 482-655 | 482-655 | Talin's VBS1 (autoinhibited 5-helix bundle) | available | Liddington |
| TLN1 | mouse | Talin / PIPKIg - soluble/great yield | pET15b-talin 482-789 | 482-789 | Talin rod fragment | available | Liddington |
| TLN1 | mouse | Talin / PIPKIg - soluble/great yield | pET151-DTOPO talin 482-889 | 482-889 | Talin rod fragment | available | Liddington |
| TLN1 | mouse | Talin / PIPKIg - soluble/great yield | pET151-DTOPO talin 482-889TTTT | 482-889 | Talin 482-889 stabilized fragment quadruple mutant (T775V/T809I/T833I/T867V) | available | Liddington |
| TLN1 | mouse | Talin / PIPKIg - soluble/great yield | pET15b-talin 755-889 | 755-889 | Talin's Vinculin-binding domain 2 (VBS2) | available | Liddington |
| TLN1 | mouse | Talin / PIPKIg - soluble/great yield | pET15b-talin 1647-2541 | 1647-2541 | Talin C-terminal fragment | available | Liddington |
| TLN1 | mouse | Talin / PIPKIg - soluble/good yield | pET151-DTOPO talin 1843-1973 | 1843-1973 | Talin VBS2 | available | Liddington |
| TLN1 | chicken | Talin / PIPKIg - soluble/great yield | pET15b-His-F2F3short | 209-400 | Talin F2F3 fused to PIPKIgamma tail | - |
Liddington |
| TLN1 | chicken | Talin - soluble/ok yield | pET15b-His-Talin Head | 1-400 | Talin Head domain | available | Liddington |
| TLN1 | chicken | Talin - soluble/great yield | pET15b-His-F2F3 | 196-400 | Talin FERM domain (F2F3 portion) | available | Liddington |
| TLN1 | chicken | Talin / PIPKIg - soluble/ok yield | pMAL-cgx-F3,4 | 309-405 | Talin FERM F3 portion | - |
Liddington |
| TLN1 | chicken | Talin / PIPKIg - low yield | pET15b-His-F3 | 309-405 | Talin FERM F3 portion | available | Liddington |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| NP_006280 | NM_006289 | Q9Y490 |
bold indicates the primary isoform
Pathways