MAP2K1 human: mitogen-activated protein kinase kinase 1
Aliases: protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1), mitogen-activated protein kinase kinase 1, MEK1, MAP2K1, MKK1, MAPKK1, PRKMK1
Pfam Domain Structure
From MAP2K1 (human)
Protein Overview
| Official Gene Name | MAP2K1 (H. sapiens) |
| RefSeq Protein Name | mitogen-activated protein kinase kinase 1 |
| UniProt Gene Symbol | MP2K1_HUMAN |
| UniProt Name | Dual specificity mitogen-activated protein kinase kinase 1 |
| Enzyme Commission | 2.7.12.2 |
| Entrez Gene Summary | The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein (MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon wide variety of extra- and intracellular signals. As an essential component of MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. [provided by RefSeq] |
References
- Dhanasekaran DN, Kashef K, Lee CM, Xu H, Reddy EP. Scaffold proteins of MAP-kinase modules. Oncogene 2007; 26:3185-202 (PubMed)
- Nakamura K, Uhlik MT, Johnson NL, Hahn KM, Johnson GL. PB1 domain-dependent signaling complex is required for extracellular signal-regulated kinase 5 activation. Mol. Cell. Biol. 2006; 26:2065-79 (PubMed)
- Nishina H, Wada T, Katada T. Physiological roles of SAPK/JNK signaling pathway. J. Biochem. 2004; 136:123-6 (PubMed)
- Wang X, Destrument A, Tournier C. Physiological roles of MKK4 and MKK7: insights from animal models. Biochim. Biophys. Acta 2007; 1773:1349-57 (PubMed)
- Whitmarsh AJ, Davis RJ. Role of mitogen-activated protein kinase kinase 4 in cancer. Oncogene 2007; 26:3172-84 (PubMed)
Consortium Results & Data
Discovery» Drosophila Migration Genes
| Gene | Name | Function |
Cell Type |
Phenotypes |
Reagents/ Alleles |
Reference |
|
Dsor1 Entrez: CG15793 Flybase: FBgn0010269 |
Downstream of Raf | serine/threonine kinase activity, MAP kinase kinase activity; receptor signaling protein | embryonic trachea | Loss of function: trachea migration defect | Dsor1Gp158 | Mayuko Nishimura, 2007 |
| border cells |
Loss of function: incomplete border cell migration Autonomous: autonomous Gain of function: not reported Comments: downstream of Raf |
DsorLH110 | Bianco 2007 |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
Dsor1 Flybase Entrez CMCKB |
Downstream of raf1 |
Mig: 187.2 Non-Mig: 231.8 |
0.80762 | 154939_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG15793 Entrez Gene: 31872 |
Downstream of raf1 Aliases: Drosophila MEK; Map kinase kinase; MAPK kinase; downstream suppressor of Raf 1 |
2.286 0.267 |
1.548 | 0.996 |
Actin: 1.626 Area: 1.2 Ratio: 1.355 |
|
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
MAP2K1 Aliases: MEK1, MKK1, MAPKK1, PRKMK1 Entrez Gene: 5604 siRNA catalog |
mitogen-activated protein kinase kinase 1 mRNA: NM_002755 Library: Kinase Classification: Ser/Thr Kinase |
Final bin: No Phenotype SMARTpool bin: No change Avg area: 1.63 Avg Alamar: 1.02 Knockdown %: 80-90 |
 Link |
 Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
MAP2K1 Aliases: MEK1, MKK1, MAPKK1, PRKMK1 Entrez Gene: 5604 siRNA catalog |
mitogen-activated protein kinase kinase 1 mRNA: NM_002755 Library: Kinase |
 Link |
 Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00219604 | MAP2K1 | DUAL SPECIFICITY MITOGEN-ACTIVATED PROTEIN KINASE KINASE 1. | 29 | 9 | 41 | 10 | 1.41 |
Consortium Related Products*
Constructs » activity page
| Ortholog | Species | Targets | Name | Amino Acid Range | Portion | Protocol | Source |
|---|---|---|---|---|---|---|---|
| MAP2K1 | human | MEK1 | pRSETA-MEKK1 | ? | Full-length MEK1 | - |
Liddington |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.