Trio mouse: triple functional domain (PTPRF interacting)
Aliases: triple functional domain (PTPRF interacting), Trio, 6720464I07Rik, AA408740, Solo
Pfam Domain Structure
From Trio (mouse)
Protein Overview
| Official Gene Name | Trio (M. musculus) |
| RefSeq Protein Name | triple functional domain (PTPRF interacting) |
| UniProt Gene Symbol | TRIO_MOUSE |
| UniProt Name | Triple functional domain protein |
References
- Chhatriwala MK, Betts L, Worthylake DK, Sondek J. The DH and PH domains of Trio coordinately engage Rho GTPases for their efficient activation. J. Mol. Biol. 2007; 368:1307-20 (PubMed)
- Henson PM. Engulfment: ingestion and migration with Rac, Rho and TRIO. Curr. Biol. 2005; 15:R29-30 (PubMed)
- Medley QG, Buchbinder EG, Tachibana K, Ngo H, Serra-Pagès C, Streuli M. Signaling between focal adhesion kinase and trio. J. Biol. Chem. 2003; 278:13265-70 (PubMed)
- Steven R, Zhang L, Culotti J, Pawson T. The UNC-73/Trio RhoGEF-2 domain is required in separate isoforms for the regulation of pharynx pumping and normal neurotransmission in C. elegans. Genes Dev. 2005; 19:2016-29 (PubMed)
Consortium Results & Data
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
TRIO Aliases: tgat Entrez Gene: 7204 siRNA catalog |
triple functional domain (PTPRF interacting) mRNA: NM_007118 Library: Kinase Classification: Ser/Thr Kinase;Tyr Kinase Focal Adhesion Related: Yes |
Final bin: Discordant SMARTpool bin: Accelerated Avg area: 0.53 Avg Alamar: 0.99 Knockdown %: 80-90 |
ERBB2: Accelerated |
&lib=Kinase&fbin=Discordant) Link |
&lib=Kinase&fbin=Discordant) Link |
Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
TRIO Aliases: tgat Entrez Gene: 7204 siRNA catalog |
triple functional domain (PTPRF interacting) mRNA: NM_007118 Library: Kinase |
&lib=Kinase) Link |
&lib=Kinase) Link |
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00442035 | TRIO | HYPOTHETICAL PROTEIN FLJ16543. | 0 | 0 | 2 | 1 | PDU |
| IPI00479523 | TRIO | SPLICE ISOFORM 2 OF TRIPLE FUNCTIONAL DOMAIN PROTEIN. | 5 | 3 | 6 | 5 | 1.20 |
Discovery» C. elegans genes affecting migration
| Gene | Description/Function | Parental P0 defect |
Distribution1 (percent) |
Progeny F1 defect |
Distribution2 (percent) |
Express DTC | Express BWM |
|
Gene: unc‑73 (F55C7.7) Gene Pair ID CMKB Entrez Gene |
UNCoordinated AhringerAutoClass: Signaling Function: Signaling InterPro: Guanyl-nucleotide exchange factor activity |
0.32 (N=62) 0.21 – 0.45 |
Type 1: 0 Type 2: 0 Type 3: 100 |
0.19 (N=64) 0.1 – 0.3 |
Type 1: 0 Type 2: 0 Type 3: 100 |
Yes
|
Yes
|
Discovery» MTLn3 invasive data
| Gene | mRNA | Description | Expression Ratio |
|
Trio Entrez Gene: 223435 |
mRNA: AW547945 UniGene: Mm.211229 |
Triple functional domain (PTPRF interacting) | 3.2 |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG18214 Entrez Gene: 43974 |
trio |
15.250 1.217 |
0.982 | 0.992 |
Actin: 0.865 Area: 1.045 Ratio: 0.828 |
|
|
CG18214 Entrez Gene: 43974 |
trio |
9.188 1.366 |
1.268 | 1.153 |
Actin: 1.073 Area: 0.882 Ratio: 1.217 |
|
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| TRIO | mouse | Trio | - |
Embryonic lethality between E15.5 and birth. Abnormal skeletal myofibers and aberrant organization in the hippocampus and olfactory bulb. | completed |
-
|
-
|
O'Brien et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| NP_001074771 | NM_001081302 | Q0KL02 |
bold indicates the primary isoform
Pathways