Nck1 mouse: non-catalytic region of tyrosine kinase adaptor protein 1
Aliases: non-catalytic region of tyrosine kinase adaptor protein 1, Nck, Nck1, 6330586M15Rik, D230010O13Rik
Protein Overview
| Official Gene Name | Nck1 (M. musculus) |
| RefSeq Protein Name | non-catalytic region of tyrosine kinase adaptor protein 1 |
References
- Baumgartner M, Sillman AL, Blackwood EM, Srivastava J, Madson N, Schilling JW, Wright JH, Barber DL. The Nck-interacting kinase phosphorylates ERM proteins for formation of lamellipodium by growth factors. Proc. Natl. Acad. Sci. U.S.A. 2006; 103:13391-6 (PubMed)
- Blasutig IM, New LA, Thanabalasuriar A, Dayarathna TK, Goudreault M, Quaggin SE, Li SS, Gruenheid S, Jones N, Pawson T. Phosphorylated YDXV motifs and Nck SH2/SH3 adaptors act cooperatively to induce actin reorganization. Mol. Cell. Biol. 2008; 28:2035-46 (PubMed)
- Buday L, Wunderlich L, Tamás P. The Nck family of adapter proteins: regulators of actin cytoskeleton. Cell. Signal. 2002; 14:723-31 (PubMed)
- Li W, Fan J, Woodley DT. Nck/Dock: an adapter between cell surface receptors and the actin cytoskeleton. Oncogene 2001; 20:6403-17 (PubMed)
- Rivera GM, Antoku S, Gelkop S, Shin NY, Hanks SK, Pawson T, Mayer BJ. Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B. Proc. Natl. Acad. Sci. U.S.A. 2006; 103:9536-41 (PubMed)
Consortium Results & Data
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
NCK1 Aliases: NCK, NCKalpha Entrez Gene: 4690 siRNA catalog |
NCK adaptor protein 1 mRNA: NM_006153 Library: MAR Classification: adaptor |
Final bin: Accelerated - MC SMARTpool bin: Accelerated Avg area: 0.27 Avg Alamar: 1.04 Knockdown %: not done |
ERBB2: Accelerated |
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Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
NCK1 Aliases: NCK, NCKalpha Entrez Gene: 4690 siRNA catalog |
NCK adaptor protein 1 mRNA: NM_006153 Library: MAR |
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Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00028065 | NCK1 | CYTOPLASMIC PROTEIN NCK1. | 1 | 1 | 0 | 0 | 0.00 |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
dock Flybase Entrez CMCKB |
dreadlocks |
Mig: 118.4 Non-Mig: 82.1 |
1.44210 | 142879_at |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| NCK1 | mouse | Nck1 / Nck2 - embryonic fibroblasts | - |
Defects in cell motility and organization of the lamellipodial actin network. | completed |
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Bladt et al. |
| NCK1 | mouse | Nck1 / Nck2 | - |
Nck genes have broad and overlapping expression pattern and mice deficient in either Nck1 or Nck2 are viable, whereas inactivation of both resulted in profound defects in mesoderm-derived notochord and embryonic lethality at day 9.5. | completed |
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Bladt et al. |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.