DOCK1 human: dedicator of cytokinesis 1
Aliases: dedicator of cyto-kinesis 1, dedicator of cytokinesis 1, DOCK180, DOCK1, RP11-82L9.1, ced5
Pfam Domain Structure
From DOCK1 (human)
Protein Overview
| Official Gene Name | DOCK1 (H. sapiens) |
| RefSeq Protein Name | dedicator of cytokinesis 1 |
| UniProt Gene Symbol | DOCK1_HUMAN |
| UniProt Name | Dedicator of cytokinesis protein 1 |
| Entrez Gene Summary | This gene product binds to the SH3 domain of CRK protein. It may regulate cell surface extension and may have a role in the cell surface extension of an engulfing cell around a dying cell during apoptosis. [provided by RefSeq] |
References
- Côté JF, Vuori K. Identification of an evolutionarily conserved superfamily of DOCK180-related proteins with guanine nucleotide exchange activity. J. Cell. Sci. 2002; 115:4901-13 (PubMed)
- Grimsley CM, Kinchen JM, Tosello-Trampont AC, Brugnera E, Haney LB, Lu M, Chen Q, Klingele D, Hengartner MO, Ravichandran KS. Dock180 and ELMO1 proteins cooperate to promote evolutionarily conserved Rac-dependent cell migration. J. Biol. Chem. 2004; 279:6087-97 (PubMed)
- Li W, Fan J, Woodley DT. Nck/Dock: an adapter between cell surface receptors and the actin cytoskeleton. Oncogene 2001; 20:6403-17 (PubMed)
- Lu M, Kinchen JM, Rossman KL, Grimsley C, Hall M, Sondek J, Hengartner MO, Yajnik V, Ravichandran KS. A Steric-inhibition model for regulation of nucleotide exchange via the Dock180 family of GEFs. Curr. Biol. 2005; 15:371-7 (PubMed)
- Reif K, Cyster J. The CDM protein DOCK2 in lymphocyte migration. Trends Cell Biol. 2002; 12:368-73 (PubMed)
Consortium Results & Data
Discovery» siRNA migration screen using a wound healing approach
| Gene | Description | MCF-10A phenotype | Secondary screen | Wound Image | Morphology | Time-lapse |
|
DOCK1 Aliases: ced5, DOCK180 Entrez Gene: 1793 siRNA catalog |
dedicator of cytokinesis 1 mRNA: NM_001380 Library: MAR Classification: GTPase regulator Focal Adhesion Related: Yes |
Final bin: No Phenotype SMARTpool bin: No change Avg area: 1.25 Avg Alamar: 0.93 Knockdown %: not done |
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Discovery» siRNA Focal Adhesion Phenotypes
| Gene | Description | High-Res | Montage |
|
DOCK1 Aliases: ced5, DOCK180 Entrez Gene: 1793 siRNA catalog |
dedicator of cytokinesis 1 mRNA: NM_001380 Library: MAR |
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Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
| IPI | Locus | Description | CB Spectra | CB peptide | PD Spectra | PD peptide | ratio |
| IPI00015286 | DOCK1 | DEDICATOR OF CYTOKINESIS PROTEIN 1. | 4 | 4 | 7 | 5 | 1.75 |
Proteomics» Table 2. Proteins in major organelles and subcellular compartments
| IPI | Locus | Description | CB Spectra | PD Spectra | ratio | Location |
| IPI00015286 | DOCK1 | DEDICATOR OF CYTOKINESIS PROTEIN 1. | 4 | 7 | 1.75 | cytoskeleton |
Discovery» Drosophila Migration Genes
| Gene | Name | Function |
Cell Type |
Phenotypes |
Reagents/ Alleles |
Reference |
|
mbc Entrez: CG10379 Flybase: FBgn0015513 |
myoblast city | Rac exchange factor, DOCK180 | border cells |
Loss of function: incomplete border cell migration Autonomous: autonomous Gain of function: not reported Comments: enhances reduction of elmo |
mbcC2, mbcD11.2, FRT, UAS-mbc RNAi | Duchek 2001; Bianco 2007; Geisbrecht 2008 |
| dorsal closure | Loss of function: dorsal closure defects (defects in cytoskeletal organization in the migrating epidermis) | Df(3R)mbc-30, Df(3R)mbc-15, mbcS4 | Erickson, 1997 | |||
| embryonic hemocyte |
Loss of function: no phenotype Gain of function: ND Comments: Deficiency that removes mbc has phenotype |
Df(3R)mbc-30, Df(3R)mbc-15, reported nulls: mbcC1, mbcD11.2 | Paladi 2004 |
Discovery» wt-migratory_cells vs wt-non-migratory_cells
| Symbol | Description | Median Values | Fold Change | Probe ID |
|
mbc Flybase Entrez CMCKB |
myoblast city |
Mig: 511.3 Non-Mig: 325.2 |
1.57230 | 143808_at |
Discovery» Drosophila Morphology Genes
| Gene | Name |
Cell Count/ Normalization |
Stellate11 NumCorners |
Stellate22 Binsum6 |
Non-Spreading3 | |
|
CG10379 Entrez Gene: 42817 |
myoblast city |
11.250 1.449 |
0.837 | 0.93 |
Actin: 0.962 Area: 0.849 Ratio: 1.133 |
|
Discovery» C. elegans genes affecting migration
| Gene | Description/Function | Parental P0 defect |
Distribution1 (percent) |
Progeny F1 defect |
Distribution2 (percent) |
Express DTC | Express BWM |
|
Gene: ced‑5 (C02F4.1) Gene Pair ID CMKB Entrez Gene |
CEll Death abnormality AhringerAutoClass: Cell Architecture Function: Cell Architecture InterPro: *Small GTPase regulatory/interacting protein |
0.38 (N=98) 0.28 – 0.48 |
Type 1: 16 Type 2: 27 Type 3: 57 |
0.7 (N=86) 0.59 – 0.79 |
Type 1: 7 Type 2: 46 Type 3: 47 |
Consortium Related Products*
Mice » activity page
| Ortholog | Species | Name | Method | Phenotype | Status | Jax Availability | Source | Publications |
|---|---|---|---|---|---|---|---|---|
| DOCK1 | mouse | DOCK 180 | Targeted - nonfloxed | - |
completed - mouse generated, evaluation in process |
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Burds |
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| DOCK1 | mouse | DOCK 180 | Targeted - floxed | - |
completed - mouse generated, evaluation in process |
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Burds |
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Constructs » activity page
| Ortholog | Species | Targets | Name | Amino Acid Range | Portion | Protocol | Source |
|---|---|---|---|---|---|---|---|
| DOCK1 | human | DOCK180 | pGEX-6P2 | 154-256 | DOCK180 DHR1 domain | available | Liddington |
* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.
Isoforms
| Isoform Name | RefSeq Protein | RefSeq mRNA | Swissprot ID |
|---|---|---|---|
| NP_001371 | NM_001380 | Q14185 |
bold indicates the primary isoform
Pathways