Pak Family

Aliases: p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related), p21-activated kinase 1, STE20 homolog, yeast, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast), PAKalpha, PAK1, MGC130000, MGC130001

Pfam Domain Structure

From PAK1 (human) - phosphorylation sites added via direct mapping

Members of the Pak Family
1: PAK1 » Human Mouse Rat Chicken Fly Worm
CMKB PAK1 Pak1 Pak1 PAK1 Pak pak-1
Entrez Gene 5058 18479 29431 419083 44039 180873
Uniprot PAK1_HUMAN PAK1_MOUSE PAK1_RAT - - PAK1_CAEEL
OMIM 602590 - - - - -
Reagents - - - - - -
Results 2 available 1 available - - 2 available -
2: PAK2 » Human Mouse Rat Chicken Fly Worm
CMKB PAK2 Pak2 Pak2 PAK2 - -
Entrez Gene 5062 224105 29432 424860 - -
Uniprot PAK2_HUMAN PAK2_MOUSE PAK2_RAT - - -
OMIM 605022 - - - - -
Reagents - - - - - -
Results 3 available - - - - -
3: PAK3 » Human Mouse Rat Chicken Fly Worm
CMKB PAK3 Pak3 Pak3 PAK3 - -
Entrez Gene 5063 18481 29433 422342 - -
Uniprot PAK3_HUMAN PAK3_MOUSE PAK3_RAT - - -
OMIM 300142 - - - - -
Reagents - - - - - -
Results 2 available - - - - -
4: PAK4 » Human Mouse Rat Chicken Fly Worm
CMKB PAK4 Pak4 Pak4 - - -
Entrez Gene 10298 70584 292756 - - -
Uniprot PAK4_HUMAN PAK4_MOUSE - - - -
OMIM 605451 - - - - -
Reagents - - - - - -
Results 5 available - - - - -
5: PAK6 » Human Mouse Rat Chicken Fly Worm
CMKB PAK6 Pak6 Pak6 PAK6 - pak-2
Entrez Gene 56924 214230 296078 428837 - 179527
Uniprot PAK6_HUMAN PAK6_MOUSE - - - -
OMIM 608110 - - - - -
Reagents - - - - - -
Results 2 available - - - - -
6: PAK7 » Human Mouse Rat Chicken Fly Worm
CMKB PAK7 Pak7 Pak7 - mbt -
Entrez Gene 57144 241656 311450 - 32631 -
Uniprot PAK7_HUMAN PAK7_MOUSE - - PAKM_DROME -
OMIM 608038 - - - - -
Reagents - - - - - -
Results 2 available - - - 1 available -
Family Summary

PAK (p21-activated kinase) is a serine/threonine kinase that binds to and is activated by Rho family GTPases. The PAK family of kinases is highly conserved among species. PAK family members have an N-terminal kinase domain and a C-terminal p21 Rac and Cdc-42-binding domain. The binding of Rho family GTPases to the C-terminus of PAK causes a conformational change resulting in autophosphorylation and activation of the kinase. PAK family members have been shown to play a role in actin polymerization and cytoskeletal dynamics. The association between NCK and PAK1 has been shown to occur through the first N-terminal polyproline domain of PAK1 and an SH3 domain of NCK. SLP-76 has been shown to provide a scaffold for the assembly of NCK/PAK1 in close proximity with VAV and RAC leading to activation of PAK1 and subsequent downstream cytoskeletal events.

References
  1. Bokoch GM. Biology of the p21-activated kinases. Annu. Rev. Biochem. 2003; 72:743-81 (PubMed)
  2. Chew TL, Masaracchia RA, Goeckeler ZM, Wysolmerski RB. Phosphorylation of non-muscle myosin II regulatory light chain by p21-activated kinase (gamma-PAK). J. Muscle Res. Cell. Motil. 1998; 19:839-54 (PubMed)
  3. Cotteret S, Chernoff J. Pak GITs to Aurora-A. Dev. Cell 2005; 9:573-4 (PubMed)
  4. Dummler B, Ohshiro K, Kumar R, Field J. Pak protein kinases and their role in cancer. Cancer Metastasis Rev. 2009; 28:51-63 (PubMed)
  5. Fryer BH, Field J. Rho, Rac, Pak and angiogenesis: old roles and newly identified responsibilities in endothelial cells. Cancer Lett. 2005; 229:13-23 (PubMed)
  6. Gerthoffer WT. Actin cytoskeletal dynamics in smooth muscle contraction. Can. J. Physiol. Pharmacol. 2005; 83:851-6 (PubMed)
  7. Hoefen RJ, Berk BC. The multifunctional GIT family of proteins. J. Cell. Sci. 2006; 119:1469-75 (PubMed)
  8. Hofmann C, Shepelev M, Chernoff J. The genetics of Pak. J. Cell. Sci. 2004; 117:4343-54 (PubMed)
  9. Howe AK. Regulation of actin-based cell migration by cAMP/PKA. Biochim. Biophys. Acta 2004; 1692:159-74 (PubMed)
  10. Juliano RL, Reddig P, Alahari S, Edin M, Howe A, Aplin A. Integrin regulation of cell signalling and motility. Biochem. Soc. Trans. 2004; 32:443-6 (PubMed)
  11. Labruyère E, Guillén N. Host tissue invasion by Entamoeba histolytica is powered by motility and phagocytosis. Arch. Med. Res. 2006; 37:253-8 (PubMed)
  12. Manser E, Leung T, Salihuddin H, Zhao ZS, Lim L. A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 1994; 367:40-6 (PubMed)
  13. Parrini MC, Matsuda M, de Gunzburg J. Spatiotemporal regulation of the Pak1 kinase. Biochem. Soc. Trans. 2005; 33:646-8 (PubMed)
  14. Settleman J. PAK-in' up cGMP for the move. Cell 2007; 128:237-8 (PubMed)
  15. Vlahou G, Rivero F. Rho GTPase signaling in Dictyostelium discoideum: insights from the genome. Eur. J. Cell Biol. 2006; 85:947-59 (PubMed)
  16. Zhao ZS, Manser E. PAK and other Rho-associated kinases--effectors with surprisingly diverse mechanisms of regulation. Biochem. J. 2005; 386:201-14 (Full Text)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
PAK1 none binding partner Pak1 Ectopic expression in HEK cells 13 proteins exclusively co-purified with Pak1 Horwitz Mayhew et al.
PAK1 none phosphorylation site Pak1 Ectopic expression in HEK cells 30 phospho-sites in 80.5% coverage of 529 aa Horwitz Mayhew et al.

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Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG18582
Entrez Gene: 32631
mushroom bodies tiny
Aliases: anon-fast-evolving-1G7
11.063
0.850
0.931 0.952 Actin: 1.024
Area: 1.182
Ratio: 0.866

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Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG10295
Entrez Gene: 44039
PAK-kinase
Aliases: Pak1 kinase; p21-associated kinase; p-21 activated kinase; p21-activated kinase; Pak kinase; PAK kinase; p21-activated serine/threonine kinase
10.125
1.138
0.99 0.989 Actin: 0.871
Area: 0.948
Ratio: 0.919
Consortium Related Products*
Biosensors » activity page
Ortholog Species Name Type Source Publications
PAK1 none PAK Generically encoded caged PAK inhibitor Hahn
-

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.

The primary classification of CMKB families is the homeomorphic family, whose members are both homologous (evolved from a common ancestor) and homeomorphic (sharing full-length sequence similarity and a common domain architecture).