Paxillin Family

Aliases: paxillin, PXN, hCG_1778014, FLJ16691

Pfam Domain Structure

From PXN (chicken) - phosphorylation sites added via direct mapping

Members of the Paxillin Family
1: PXN » Human Mouse Rat Chicken Fly Worm
CMKB PXN Pxn Pxn PXN Pax pxl-1
Entrez Gene 5829 19303 360820 395832 35215 175831
Uniprot PAXI_HUMAN PAXI_MOUSE PAXI_RAT PAXI_CHICK - PXL1_CAEEL
OMIM 602505 - - - - -
Reagents 2 available 4 available - - - -
Results 3 available - - - 2 available -
2: LPXN » Human Mouse Rat Chicken Fly Worm
CMKB LPXN Lpxn Lpxn LPXN - -
Entrez Gene 9404 107321 293783 423129 - -
Uniprot LPXN_HUMAN LPXN_MOUSE - - - -
OMIM 605390 - - - - -
Reagents 1 available - - - - -
Results - - - - - -
Family Summary

Paxillin is a multi-domain protein that localizes to focal adhesions. The C-terminal region of paxillin contains four LIM domains, which target paxillin to focal adhesions, presumably through a direct association with the cytoplasmic tail of beta-integrin. The N-terminal region of paxillin is rich in protein-protein interaction sites. The proteins that bind to paxillin are diverse and include protein tyrosine kinases, such as Src and FAK, structural proteins, such as vinculin and actopaxin, and regulators of actin organization, such as COOL/PIX and PKL/GIT. Paxillin is tyrosine-phosphorylated by FAK and Src upon integrin engagement or growth factor stimulation, creating binding sites for the adapter protein Crk. Leupaxin is preferentially expressed in hematopoietic cells and is homologous to paxillin. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by tyrosine kinase activity.

References
  1. Chen PW, Kroog GS. Leupaxin is similar to paxillin in focal adhesion targeting and tyrosine phosphorylation but has distinct roles in cell adhesion and spreading. Cell Adh Migr 2010; 4: (Full Text)
  2. Deakin NO, Turner CE. Paxillin comes of age. J. Cell. Sci. 2008; 121:2435-44 (Full Text)
  3. Hoefen RJ, Berk BC. The multifunctional GIT family of proteins. J. Cell. Sci. 2006; 119:1469-75 (PubMed)
  4. Huang C, Jacobson K, Schaller MD. A role for JNK-paxillin signaling in cell migration. Cell Cycle 2004; 3:4-6 (PubMed)
  5. Rathore VB, Okada M, Newman PJ, Newman DK. Paxillin family members function as Csk-binding proteins that regulate Lyn activity in human and murine platelets. Biochem. J. 2007; 403:275-81 (Full Text)
  6. Rose DM. The role of the alpha4 integrin-paxillin interaction in regulating leukocyte trafficking. Exp. Mol. Med. 2006; 38:191-5 (PubMed)
  7. Sabe H, Onodera Y, Mazaki Y, Hashimoto S. ArfGAP family proteins in cell adhesion, migration and tumor invasion. Curr. Opin. Cell Biol. 2006; 18:558-64 (PubMed)
  8. Schaller MD. FAK and paxillin: regulators of N-cadherin adhesion and inhibitors of cell migration?. J. Cell Biol. 2004; 166:157-9 (Full Text)
  9. Spinardi L, Marchisio PC. Podosomes as smart regulators of cellular adhesion. Eur. J. Cell Biol. 2006; 85:191-4 (PubMed)
  10. Webb DJ, Donais K, Whitmore LA, Thomas SM, Turner CE, Parsons JT, Horwitz AF. FAK-Src signalling through paxillin, ERK and MLCK regulates adhesion disassembly. Nat. Cell Biol. 2004; 6:154-61 (PubMed)
Consortium Results & Data
Results & Data
Ortholog Species Type Description Method Status Source Publications
PXN none binding partner Paxillin Ectopic expression in HEK cells 10 co-purifying proteins validated Horwitz Schroeder et al., Mayhew et al.
PXN none phosphorylation site Paxillin Ectopic expression in HEK cells 38 phospho-sites in 79.4% coverage of 791 aa Horwitz Schroeder et al., Webb et al., Nayal et al.
PXN none structure

Paxillin -wt fragments 1-328 & 325-557 and full-length

X-Ray crystallography

completed Liddington
-
PXN none structure

Paxillin (1-328)-FAT domain of FAK

NMR

completed Campbell
-
Discovery» wt-migratory_cells vs wt-non-migratory_cells
Symbol Description Median Values Fold Change Probe ID
CG18061
Flybase
Entrez
Paxillin Mig: 620.0
Non-Mig: 403.2
1.53770 141605_at
CG18576
Flybase
Entrez
Paxillin Mig: 145.6
Non-Mig: 174.2
0.83584 146489_at
Discovery» Drosophila Morphology Genes
Gene Name Cell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
CG31794
Entrez Gene: 35215
Paxillin 0.444
0.065
1.909 1.223 Actin: 0.651
Area: 1.239
Ratio: 0.525
Discovery» siRNA migration screen using a wound healing approach
Gene Description     MCF-10A phenotype     Secondary screen Wound Image Morphology Time-lapse
PXN
Entrez Gene: 5829
siRNA catalog
paxillin
mRNA: NM_002859
Library: MAR
Classification: cytoskeletal
Focal Adhesion Related: Yes
Final bin: Discordant
SMARTpool bin: Low Alamar
Avg area: 1.79
Avg Alamar: 0.77
Knockdown %: not done
ERBB2: Impaired
BCL2: Low Alamar
PXN
Link
PXN
Link
 
Discovery» siRNA Focal Adhesion Phenotypes
Gene Description High-Res Montage
PXN
Entrez Gene: 5829
siRNA catalog
paxillin
mRNA: NM_002859
Library: Control
PXN
Link
PXN
Link
Proteomics» Table 1. Identification and Quantitation of Proteins from Cell Body (CB) and Pseudopodium (PD) fractions
IPI Locus Description CB Spectra CB peptide PD Spectra PD peptide ratio
IPI00220030 PXN SPLICE ISOFORM ALPHA OF PAXILLIN. 14 9 20 13 1.43
Consortium Related Products*
Mice » activity page
Ortholog Species Name Method Phenotype Status Jax Availability Source Publications
PXN mouse Paxillin - cultured ES cells
-
Delayed spreading on fibronectin and laminin, reduced tyrosine phosphorylation of Fak. completed
-
-
Wade et al.
PXN mouse Paxillin - fibroblasts
-
Abnormal focal adhesions, reduced cell migration, inefficient localization of Fak, defects in cortical cytoskeleton and cell spreading on fibronectin. completed
-
-
Hagel et al.
PXN mouse Paxillin conditional expression of FLAG-mCherry-Paxillin
-
completed - mouse available
-
Ginsberg
-
PXN mouse Paxillin
-
Embryonic lethality due to abnormal development of mesodermally derived structures such as the heart and somites. completed
-
-
Hagel et al.
Constructs » activity page
Ortholog Species Targets Name Amino Acid Range Portion Protocol Source
PXN human Paxillin - mut in Histag,protein not in frame with start pET28-paxillin 1-557 Paxillin -full length
-
Liddington
PXN human Paxillin - not soluble poor yield pGEX4T2-paxillin 38-557 38-557 Paxillin (N-terminal truncated)
-
Liddington
LPXN human Leupaxin pET15b-leupaxin 1-386 Leupaxin -full length
-
Ginsberg
Biosensors » activity page
Ortholog Species Name Type Source Publications
PXN none paxillin - (Tyr31) semisynthetic mutants Imperiali Vogel and Imperiali
PXN none paxillin - (Tyr31) semisynthetic mutants Imperiali
-
PXN none paxillin - (peptide) Environment sensitive fluorophore Imperiali Vogel and Imperiali
PXN none paxillin - (Tyr31) Environment sensitive fluorophore Imperiali
-

* The resources presented here are largely those generated by the Consortium. The sidebar provides public databases that complement Consortium activities. For mice and biosensors, a public database is not available and therefore we have attempted to generate a migration related list for your convenience.

The primary classification of CMKB families is the homeomorphic family, whose members are both homologous (evolved from a common ancestor) and homeomorphic (sharing full-length sequence similarity and a common domain architecture).